CAJ | 학술논문

目的：对2009年从中国云南省1名脑炎患儿粪便标本中分离到的Echo30云南分离株KM／A363／09进行全基因组测序和分析，了解该株病毒的遗传特性。方法设计针对Echo30引物，提取病毒RNA、RT-PCR扩增和产物直接测序获得全基因组序列，利用Geneious和Mega5．1软件进行核苷酸、氨基酸序列比对及其系统进化分析，应用RDP3和SimPlot3．5．1重组软件对序列进行重组分析。结果该分离株全基因组核苷酸序列长度为7425 bp，编码2194个氨基酸。其核苷酸和氨基酸序列与其原型株Bastianni的同源性分别为81．2％和95．8％；与其他Echo30型毒株的各区段核苷酸和氨基酸同源性分别为81．2％～88．6％和95．8％～97．8％；进化分析发现KM／A363／09毒株属于中国Echo30分支中的一支。而且发现KM／A363／09株基因序列在非结构区可能存在重组事件。结论云南分离株KM／A363／09属于中国Echo30分支中的一支，而中国分离株已经发生一定的进化。
목적：대2009년종중국운남성1명뇌염환인분편표본중분리도적Echo30운남분리주KM／A363／09진행전기인조측서화분석，료해해주병독적유전특성。방법설계침대Echo30인물，제취병독RNA、RT-PCR확증화산물직접측서획득전기인조서렬，이용Geneious화Mega5．1연건진행핵감산、안기산서렬비대급기계통진화분석，응용RDP3화SimPlot3．5．1중조연건대서렬진행중조분석。결과해분리주전기인조핵감산서렬장도위7425 bp，편마2194개안기산。기핵감산화안기산서렬여기원형주Bastianni적동원성분별위81．2％화95．8％；여기타Echo30형독주적각구단핵감산화안기산동원성분별위81．2％～88．6％화95．8％～97．8％；진화분석발현KM／A363／09독주속우중국Echo30분지중적일지。이차발현KM／A363／09주기인서렬재비결구구가능존재중조사건。결론운남분리주KM／A363／09속우중국Echo30분지중적일지，이중국분리주이경발생일정적진화。
Objective To analyze the genetic characteristics of the complete genome of a human echovirus 30 (Echo30) KM/A363/09 strain isolated in Yunnan, China in 2009.Methods Primers specif-ic for Echo30 were designed .The extracted RNA was amplified by using RT-PCR.Seven fragments covering the complete viral genome were sequenced and the complete sequences were aligned with other sequences of enterovirus reference strains downloaded from Genbank . By using Mega5.1, Geneious, RDP3 and SimPlot3.5.1 softwares, the phylogenetic and recombination analysis were carried out .Results The com-plete nucleotide sequence of KM/A363/09 isolate was 7425 bp in length, encoding 2194 amino acids.KM/A363/09 isolate was highly similar with Bastianni prototype strain showing the homology of 81.2%in nucle-otide and 95.8%in amino acid.The eight Echo30 isolates shared 81.2%-88.6%homologies in nucleotide sequences and 95.8%-97.8%in amino acid sequences .Phylogenetic analysis showed that the KM/A363/09 strain belonged to one clade of Echo 30 in China.The genetic recombination of KM/A363/09 isolate was detected in the non-structural region .Conclusion KM/A363/09 isolate belongs to one clade of Echo 30 in China indicating that the evolution of Echo 30 has occurred in China .