CAJ | 학술논문

目的：探索大肠埃希菌O157∶H7中志贺毒素噬菌体对Ⅲ型分泌系统表达的影响。方法本研究采用了大肠埃希菌标准菌株EDL933与EDL志贺毒素噬菌体天然缺失株TUV93-0进行Ⅲ型分泌系统蛋白表达比对，而后利用表达Ⅲ型分泌系统各个操纵子（LEE1～LEE5）的荧光质粒，检测各个操纵子的表达情况。结果与野生株EDL933相比，志贺毒素噬菌体缺失株中的Ⅲ型分泌系统蛋白表达量明显增加，而进一步的实验证实操纵子LEE1、LEE2、LEE5的表达均有增加。结论志贺毒素噬菌体缺失后对Ⅲ型分泌系统造成影响，此种影响有可能是通过LEE1传递的。
목적：탐색대장애희균O157∶H7중지하독소서균체대Ⅲ형분비계통표체적영향。방법본연구채용료대장애희균표준균주EDL933여EDL지하독소서균체천연결실주TUV93-0진행Ⅲ형분비계통단백표체비대，이후이용표체Ⅲ형분비계통각개조종자（LEE1～LEE5）적형광질립，검측각개조종자적표체정황。결과여야생주EDL933상비，지하독소서균체결실주중적Ⅲ형분비계통단백표체량명현증가，이진일보적실험증실조종자LEE1、LEE2、LEE5적표체균유증가。결론지하독소서균체결실후대Ⅲ형분비계통조성영향，차충영향유가능시통과LEE1전체적。
Objective To investigate the effects of shiga toxin ( Stx ) phage on the expression of type Ⅲsecretion system (T3SS) in E.coli O157 ∶H7.Methods A standard E.coli O157 ∶H7 strain, EDL933 and a natural Stx phage defective mutant of EDL 933 strain, TUV93-0 were used for this study .The expression of T3SS proteins was compared between EDL 933 and TUV93-0 strains.The expression of five operons ( LEE1-LEE5 ) was evaluated by measuring the green fluorescent protein ( GFP ) in five different plasmids with LEE1-LEE5 promoters, respectively.Results The expression of T3SS proteins in TUV93-0 mutant were significantly increased than those in EDL 933 strain.Moreover, the expression of LEE1, LEE2 and LEE5 were also increased in TUV93-0 mutant.Conclusion The deletion of Stx phage might enhance T3SS expression through the regulation of LEE 1.