中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2014年
5期
359-363
,共5页
严重发热伴血小板减少综合征%新型布尼亚病毒%淋巴细胞亚群%白细胞介素-2受体
嚴重髮熱伴血小闆減少綜閤徵%新型佈尼亞病毒%淋巴細胞亞群%白細胞介素-2受體
엄중발열반혈소판감소종합정%신형포니아병독%림파세포아군%백세포개소-2수체
Severe fever with thrombocytopenia syndrome%Severe fever with thrombocytopenia syndrome bunyavirus%Lymphocyte subset%Interleukin-2 receptor
目的：探讨舟山海岛地区严重发热伴血小板减少综合征（ severe fever with thrombocy-topenia syndrome，SFTS）患者淋巴细胞亚群及白细胞介素-2受体（CD25）表达的特征。方法采用血细胞分析仪、全自动生化仪及流式细胞仪分别检测SFTS患者治疗前及治疗10 d后外周血血常规、血生化、淋巴细胞亚群，统计学分析其动态变化及相关关系。结果 SFTS患者治疗前白细胞、血小板、CD3＋T细胞、CD4＋T细胞均明显低于正常体检者。治疗10 d后，白细胞、血小板均明显上升， CD3＋T细胞、CD4＋T细胞仍低于正常体检者， CD25＋细胞高于正常体检者，差异有统计学意义（ P＜0．05）；SFTS死亡组T细胞、CD4＋T细胞、CD8＋T细胞和CD25＋细胞均显著低于痊愈组，差异有统计学意义（ P＜0．05）。 CD25＋细胞和B细胞与白细胞（WBC）呈正相关，血小板（blood platelet，PLT）与CD4＋T细胞呈正相关，差异有统计学意义（P＜0．05）；谷丙转氨酶（ALT）、肌酸激酶（CK）与CD3＋、CD8＋T细胞呈正相关，差异有统计学意义（P＜0．05）。结论新型布尼亚病毒（SFTSV）感染后机体免疫细胞比例发生改变，尤其是CD4＋T细胞减少，并与WBC、PLT和多种血清酶有一定的相关关系。
目的：探討舟山海島地區嚴重髮熱伴血小闆減少綜閤徵（ severe fever with thrombocy-topenia syndrome，SFTS）患者淋巴細胞亞群及白細胞介素-2受體（CD25）錶達的特徵。方法採用血細胞分析儀、全自動生化儀及流式細胞儀分彆檢測SFTS患者治療前及治療10 d後外週血血常規、血生化、淋巴細胞亞群，統計學分析其動態變化及相關關繫。結果 SFTS患者治療前白細胞、血小闆、CD3＋T細胞、CD4＋T細胞均明顯低于正常體檢者。治療10 d後，白細胞、血小闆均明顯上升， CD3＋T細胞、CD4＋T細胞仍低于正常體檢者， CD25＋細胞高于正常體檢者，差異有統計學意義（ P＜0．05）；SFTS死亡組T細胞、CD4＋T細胞、CD8＋T細胞和CD25＋細胞均顯著低于痊愈組，差異有統計學意義（ P＜0．05）。 CD25＋細胞和B細胞與白細胞（WBC）呈正相關，血小闆（blood platelet，PLT）與CD4＋T細胞呈正相關，差異有統計學意義（P＜0．05）；穀丙轉氨酶（ALT）、肌痠激酶（CK）與CD3＋、CD8＋T細胞呈正相關，差異有統計學意義（P＜0．05）。結論新型佈尼亞病毒（SFTSV）感染後機體免疫細胞比例髮生改變，尤其是CD4＋T細胞減少，併與WBC、PLT和多種血清酶有一定的相關關繫。
목적：탐토주산해도지구엄중발열반혈소판감소종합정（ severe fever with thrombocy-topenia syndrome，SFTS）환자림파세포아군급백세포개소-2수체（CD25）표체적특정。방법채용혈세포분석의、전자동생화의급류식세포의분별검측SFTS환자치료전급치료10 d후외주혈혈상규、혈생화、림파세포아군，통계학분석기동태변화급상관관계。결과 SFTS환자치료전백세포、혈소판、CD3＋T세포、CD4＋T세포균명현저우정상체검자。치료10 d후，백세포、혈소판균명현상승， CD3＋T세포、CD4＋T세포잉저우정상체검자， CD25＋세포고우정상체검자，차이유통계학의의（ P＜0．05）；SFTS사망조T세포、CD4＋T세포、CD8＋T세포화CD25＋세포균현저저우전유조，차이유통계학의의（ P＜0．05）。 CD25＋세포화B세포여백세포（WBC）정정상관，혈소판（blood platelet，PLT）여CD4＋T세포정정상관，차이유통계학의의（P＜0．05）；곡병전안매（ALT）、기산격매（CK）여CD3＋、CD8＋T세포정정상관，차이유통계학의의（P＜0．05）。결론신형포니아병독（SFTSV）감염후궤체면역세포비례발생개변，우기시CD4＋T세포감소，병여WBC、PLT화다충혈청매유일정적상관관계。
Objective To analyze the characteristics of lymphocyte subsets and interleukin-2 re-ceptor (CD25) in patients with severe fever with thrombocytopenia syndrome (SFTS) in Zhoushan island. Methods Automated blood analyzer , automatic biochemical analyzer and flow cytometry were used to meas-ure hematological parameters , biochemical parameters and lymphocyte subsets in patients with SFTS before and after treatment .The dynamic changes and correlation analysis were statistically analyzed .Results The counts of white blood cells (WBCs), platelets, total T cells and CD4+T cells in patients with SFTS before treatment were significantly lower than those in healthy subjects .The counts of WBCs and platelets were sig-nificantly increased after ten days of treatment , and the level of CD25+cells was higher than that in control group, but the level of total T cells and CD 4+T cells were still lower than those in healthy subjects ( P<0.05).Patients died of SFTS had significantly lower counts of total T cells , CD4+T cells, CD8+T cells and CD25+cells than cured patients (P<0.05).The level of CD25+cells and B cells were positively correlated with WBC level (P<0.05).Platelet level was positively correlated with the percentage of CD 4+T cells (P<0.05).The level of alanine aminotransferase and creatine kinase were positively correlated with the number of CD3+T and CD8+T cells (P<0.05).Conclusion SFTS virus infection affected the percentage of body′s immune cells, especially the level of CD4+T cells.The imbalanced immune system was correlated with the counts of WBCs , platelets and various enzymes in serum and it might be one of the factors causing extensive damage of multiple organs and tissues .
