中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2014年
5期
763-766
,共4页
慢性咽炎%耐药基因%高通量测序技术%检测
慢性嚥炎%耐藥基因%高通量測序技術%檢測
만성인염%내약기인%고통량측서기술%검측
chronic pharyngitis%drug resistant gene%high-throughput sequencing%detection
目的：探讨高通量测序技术在慢性咽炎患者耐药基因分布中的应用。方法选取2010年5月至2012年5月本院门诊收治的慢性咽炎患者360例，采集患者咽后壁分泌物，提取质粒 DNA 并进行 Solexa 高通量测序，通过比对在 NCBI 质粒基因组数据库进行物种鉴定，通过与 ARDB 数据库进行比对进行耐药基因鉴定。结果本研究共鉴定出307个质粒，分属25种病原菌，所占比例最高的为α-溶血性链球菌（12．87％），其次为副流感嗜血菌（9．86％）。通过与 ARDB 中已知的380种 ARG 比对，共有4015条序列与已知耐药基因序列一致。其中氨基糖苷类耐药基因、β内酰胺酶类耐药基因、大环内酯类耐药基因及四环素类耐药基因占较高的比例。同时我们也检测到一个多重耐药基因 lsa。结论高通量测序技术可以高效、准确地检测慢性咽炎患者中咽部细菌的耐药基因，为临床慢性咽炎治疗方案的选择提供理论基础。
目的：探討高通量測序技術在慢性嚥炎患者耐藥基因分佈中的應用。方法選取2010年5月至2012年5月本院門診收治的慢性嚥炎患者360例，採集患者嚥後壁分泌物，提取質粒 DNA 併進行 Solexa 高通量測序，通過比對在 NCBI 質粒基因組數據庫進行物種鑒定，通過與 ARDB 數據庫進行比對進行耐藥基因鑒定。結果本研究共鑒定齣307箇質粒，分屬25種病原菌，所佔比例最高的為α-溶血性鏈毬菌（12．87％），其次為副流感嗜血菌（9．86％）。通過與 ARDB 中已知的380種 ARG 比對，共有4015條序列與已知耐藥基因序列一緻。其中氨基糖苷類耐藥基因、β內酰胺酶類耐藥基因、大環內酯類耐藥基因及四環素類耐藥基因佔較高的比例。同時我們也檢測到一箇多重耐藥基因 lsa。結論高通量測序技術可以高效、準確地檢測慢性嚥炎患者中嚥部細菌的耐藥基因，為臨床慢性嚥炎治療方案的選擇提供理論基礎。
목적：탐토고통량측서기술재만성인염환자내약기인분포중적응용。방법선취2010년5월지2012년5월본원문진수치적만성인염환자360례，채집환자인후벽분비물，제취질립 DNA 병진행 Solexa 고통량측서，통과비대재 NCBI 질립기인조수거고진행물충감정，통과여 ARDB 수거고진행비대진행내약기인감정。결과본연구공감정출307개질립，분속25충병원균，소점비례최고적위α-용혈성련구균（12．87％），기차위부류감기혈균（9．86％）。통과여 ARDB 중이지적380충 ARG 비대，공유4015조서렬여이지내약기인서렬일치。기중안기당감류내약기인、β내선알매류내약기인、대배내지류내약기인급사배소류내약기인점교고적비례。동시아문야검측도일개다중내약기인 lsa。결론고통량측서기술가이고효、준학지검측만성인염환자중인부세균적내약기인，위림상만성인염치료방안적선택제공이론기출。
Objective To explore the application of high-throughput sequencing technology in resistance gene detec-tion in patients with chronic pharyngitis.Methods 360 cases of patients with chronic pharyngitis were selected from May 2010 to May 2012 in outpatient of our hospital.Secretion of pharyngeal wall in patients with chronic pharyngitis was collected and the plasmid was extracted for further Solexa high-throughput sequencing.Species identification was performed through mapping to NCBI plasmid genome database,and drug resistance gene identification was done by mapping to Antibiotic Resistance Database (ARDB)database.Results 307 plasmids belong to 25 kinds of pathogenic bacteria were identified,in which alpha hemolytic streptococcus accounted for the highest proportion (12.87%),and haemophellolus influenae was the second (9.86%).Through searching the plasmid metagenome for signatures of known 380 ARGs in ARDB,a total of 4015 reads were assigned to the known antibiotic resistance genes.The relative a-bundant genes included aminoglycoside resistance gene,beta lactam enzymes of resistant genes,beta-lactamases resistant genes and tetracycline resistance genes.Moreover,a multiple drug resistance gene named lsa was identified.Conclusion High-throughput sequencing technology can efficiently and accurately detect drug-resistant genes in patients with chronic pharyngitis,which provide theoretical basis for the selection of clinical treatment options in patients with chron-ic pharyngitis.
