中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2014年
5期
722-726
,共5页
尚修超%顾加祥%张乃臣%刘宏君%田恒%潘俊博
尚脩超%顧加祥%張迺臣%劉宏君%田恆%潘俊博
상수초%고가상%장내신%류굉군%전항%반준박
GDNF%骨髓间充质干细胞%周围神经%再生%诱导
GDNF%骨髓間充質榦細胞%週圍神經%再生%誘導
GDNF%골수간충질간세포%주위신경%재생%유도
GDNF%BMSCs%Peripheral nerve%Regeneration%Differentiation
目的:探讨胶质细胞源性神经营养因子(GDNF)诱导骨髓间充质干细胞(BMSCs)促周围神经再生。方法建立大鼠坐骨神经损伤模型,实验分为2组:对照组为未经诱导 BMSCs 组,实验组为 GDNF 基因诱导后 BMSCs组。每组20只 SD 大鼠。①术后4周和8周观察坐骨神经指数和腓肠肌湿质量恢复率。②术后8周测量再生神经纤维轴突直径、髓鞘厚度及有髓神经纤维计数。③术后4周腓肠肌肌细胞行 DAPI 染色。④术后8周坐骨神经扫面电镜观察神经丝再生交联情况。结果经 GDNF 基因诱导 BMSCs 组各方面检测指标均较对照组为好,其中诱导后实验组扫描电镜下观察周围神经再生更加明显。结论GDNF 基因诱导的间充质干细胞对周围神经再生有着显著的疗效。
目的:探討膠質細胞源性神經營養因子(GDNF)誘導骨髓間充質榦細胞(BMSCs)促週圍神經再生。方法建立大鼠坐骨神經損傷模型,實驗分為2組:對照組為未經誘導 BMSCs 組,實驗組為 GDNF 基因誘導後 BMSCs組。每組20隻 SD 大鼠。①術後4週和8週觀察坐骨神經指數和腓腸肌濕質量恢複率。②術後8週測量再生神經纖維軸突直徑、髓鞘厚度及有髓神經纖維計數。③術後4週腓腸肌肌細胞行 DAPI 染色。④術後8週坐骨神經掃麵電鏡觀察神經絲再生交聯情況。結果經 GDNF 基因誘導 BMSCs 組各方麵檢測指標均較對照組為好,其中誘導後實驗組掃描電鏡下觀察週圍神經再生更加明顯。結論GDNF 基因誘導的間充質榦細胞對週圍神經再生有著顯著的療效。
목적:탐토효질세포원성신경영양인자(GDNF)유도골수간충질간세포(BMSCs)촉주위신경재생。방법건립대서좌골신경손상모형,실험분위2조:대조조위미경유도 BMSCs 조,실험조위 GDNF 기인유도후 BMSCs조。매조20지 SD 대서。①술후4주화8주관찰좌골신경지수화비장기습질량회복솔。②술후8주측량재생신경섬유축돌직경、수초후도급유수신경섬유계수。③술후4주비장기기세포행 DAPI 염색。④술후8주좌골신경소면전경관찰신경사재생교련정황。결과경 GDNF 기인유도 BMSCs 조각방면검측지표균교대조조위호,기중유도후실험조소묘전경하관찰주위신경재생경가명현。결론GDNF 기인유도적간충질간세포대주위신경재생유착현저적료효。
Objective To clarify the role of Glial cell derived neurotrophic factor (GDNF)inducing bone marrow mesenchymal stem cells in promoting regeneration of peripheral nerve.Methods Sciatic nerve injury models of rats were established,Experiment was divided into two groups:experimental group was BMSCs group induced by GDNF gene,and control group was BMSCs group un-induced by GDNF gene ,each group had 20 SD rats.① Observe index of sciatic nerve and gastrocnemius wet quality recovery rate after 4 weeks and 8 weeks.② Measured axon diameter ,thick-ness of myelin sheath and myelinated nerve fiber numbers of regeneration nerve fiber 8 weeks postoperative.③The gas-trocnemius muscle cells were stained with DAPI after 4 weeks postoperative.④ Neurofilament regeneration crosslink-ing of the sciatic nerve were obsvered by sweep electron microscope after 8 weeks.Results Test index of the BMSCs group induced by the GDNF gene is better than those of the control group,peripheral nerve regeneration of experimen-tal group observed by scanning electron microscopy was better than those of control group.Conclusion bone marrow mesenchymal stem cells induced by GDNF gene have significant effection for the regeneration of peripheral nerve.