CAJ | 학술논문

目的：探讨二甲亚砜（DMSO）对外周血单个核细胞（PBMCs）增殖能力和细胞因子分泌能力的影响。方法分别设置含不同终体积分数 DMSO 的细胞培养体系及空白对照，采用羟基荧光素二醋酸盐琥珀酰亚胺脂（CFSE）示踪技术和酶联免疫斑点（ELISPOT）技术分别检测 PBMCs 的增殖能力和细胞因子分泌功能，分析 DMSO 对 PBMCs功能的影响。结果当培养体系中 DMSO 的体积分数为2％时，PBMCs 的增殖功能开始受到明显抑制，与对照组相比差异有统计学意义（P ＜0．01）；当 DMSO 的浓度为4％时，PBMCs 分泌细胞因子的功能开始受到明显抑制，与对照组相比差异有统计学意义（P ＜0．05）；并且，DMSO 对 PBMCs 增殖和细胞因子分泌功能的影响存在浓度依赖趋势。结论DMSO 浓度大于2％的培养体系会对 PBMC 产生明显的细胞毒性作用，应采取适当措施减小或规避 DMSO 对实验结果的影响。
목적：탐토이갑아풍（DMSO）대외주혈단개핵세포（PBMCs）증식능력화세포인자분비능력적영향。방법분별설치함불동종체적분수 DMSO 적세포배양체계급공백대조，채용간기형광소이작산염호박선아알지（CFSE）시종기술화매련면역반점（ELISPOT）기술분별검측 PBMCs 적증식능력화세포인자분비공능，분석 DMSO 대 PBMCs공능적영향。결과당배양체계중 DMSO 적체적분수위2％시，PBMCs 적증식공능개시수도명현억제，여대조조상비차이유통계학의의（P ＜0．01）；당 DMSO 적농도위4％시，PBMCs 분비세포인자적공능개시수도명현억제，여대조조상비차이유통계학의의（P ＜0．05）；병차，DMSO 대 PBMCs 증식화세포인자분비공능적영향존재농도의뢰추세。결론DMSO 농도대우2％적배양체계회대 PBMC 산생명현적세포독성작용，응채취괄당조시감소혹규피 DMSO 대실험결과적영향。
Objective To investigate the cytotoxicity effect of dimethyl sulfoxide (DMSO)on the proliferation and cytokine secretion of peripheral blood mononuclear cells (PBMCs).Methods PBMCs were cultured in medium with various concentrations of DMSO.CellTrace?carboxyl fluorescein diacetate succinimidyl ester (CFSE)was used as a cell proliferation tracing reagent to assess cell proliferation,and enzyme-linked immunospot (ELISPOT)assay was used to assess capability of cytokine secretion.Results Compared with the control group,the proliferation of PBMCs were in-hibited significantly (P <0.01)at the final concentrations of 2% DMSO,and the capability of cytokine secretion of PB-MCs were significantly reduced (P <0.05)at the final concentrations of 4% DMSO.In additio,the inhibition of PBMCs proliferation and cytokine secretion was consistent with the increase of DMSO concentration.Conclusion DMSO has a cytotoxicity effects on the proliferation and cytokine secretion of PBMCs,when the concentration of DMSO reaches a-bout 2% in the culture medium.