中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2014年
5期
517-520
,共4页
骨性关节炎%C2 C%Ⅱ型胶原
骨性關節炎%C2 C%Ⅱ型膠原
골성관절염%C2 C%Ⅱ형효원
Osteoarthritis%C2C%Type II collagen
目的：观察并探讨尿液中Ⅱ型胶原羧基末端3/4片段（ C2C）水平与膝关节骨性关节炎（ KOA）患者病变程度的关系。方法收集90例膝关节骨性关节炎患者及20例非膝关节骨性关节炎患者清晨尿液标本5 mL及膝关节正侧位影像学资料，尿液标本置-80℃冰箱保存，影像学资料根据Kellgren-Lawrence 放射学分级标准对膝关节骨性关节炎患者的影像学表现进行分级，实验分为五组，对照组，I级组，II级组，III级组及IV级组。各组间年龄及性别构成比较没有显著性差异，无统计学意义。尿液标本收齐后采用酶联免疫吸附试验（ELISA）检测尿液中C2C水平。结果与对照组（261.235±39.944 pg/mL）相比，膝关节骨性关节炎组C2C水平（218.341±22.270 pg/mL）明显升高，差异有统计学意义（ P＜0.01）。各组C2C水平比较：IV组（311.872±18.759 pg/mL）＞III组（273.753±18.595 pg/mL）＞II组（256.251±23.379 pg/mL）＞I组（219.009±13.431 pg/mL）或正常组（218.341±22.270 pg/mL）（P＝0.000）。 I级膝关节骨性关节炎组患者尿液C2C与对照组水平相差无统计学意义（ P＞0.05）。结论骨性关节炎患者C2C水平较正常对照组高，C2C升高水平与膝关节骨性关节炎病变程度平行，且二者呈高度正相关。
目的：觀察併探討尿液中Ⅱ型膠原羧基末耑3/4片段（ C2C）水平與膝關節骨性關節炎（ KOA）患者病變程度的關繫。方法收集90例膝關節骨性關節炎患者及20例非膝關節骨性關節炎患者清晨尿液標本5 mL及膝關節正側位影像學資料，尿液標本置-80℃冰箱保存，影像學資料根據Kellgren-Lawrence 放射學分級標準對膝關節骨性關節炎患者的影像學錶現進行分級，實驗分為五組，對照組，I級組，II級組，III級組及IV級組。各組間年齡及性彆構成比較沒有顯著性差異，無統計學意義。尿液標本收齊後採用酶聯免疫吸附試驗（ELISA）檢測尿液中C2C水平。結果與對照組（261.235±39.944 pg/mL）相比，膝關節骨性關節炎組C2C水平（218.341±22.270 pg/mL）明顯升高，差異有統計學意義（ P＜0.01）。各組C2C水平比較：IV組（311.872±18.759 pg/mL）＞III組（273.753±18.595 pg/mL）＞II組（256.251±23.379 pg/mL）＞I組（219.009±13.431 pg/mL）或正常組（218.341±22.270 pg/mL）（P＝0.000）。 I級膝關節骨性關節炎組患者尿液C2C與對照組水平相差無統計學意義（ P＞0.05）。結論骨性關節炎患者C2C水平較正常對照組高，C2C升高水平與膝關節骨性關節炎病變程度平行，且二者呈高度正相關。
목적：관찰병탐토뇨액중Ⅱ형효원최기말단3/4편단（ C2C）수평여슬관절골성관절염（ KOA）환자병변정도적관계。방법수집90례슬관절골성관절염환자급20례비슬관절골성관절염환자청신뇨액표본5 mL급슬관절정측위영상학자료，뇨액표본치-80℃빙상보존，영상학자료근거Kellgren-Lawrence 방사학분급표준대슬관절골성관절염환자적영상학표현진행분급，실험분위오조，대조조，I급조，II급조，III급조급IV급조。각조간년령급성별구성비교몰유현저성차이，무통계학의의。뇨액표본수제후채용매련면역흡부시험（ELISA）검측뇨액중C2C수평。결과여대조조（261.235±39.944 pg/mL）상비，슬관절골성관절염조C2C수평（218.341±22.270 pg/mL）명현승고，차이유통계학의의（ P＜0.01）。각조C2C수평비교：IV조（311.872±18.759 pg/mL）＞III조（273.753±18.595 pg/mL）＞II조（256.251±23.379 pg/mL）＞I조（219.009±13.431 pg/mL）혹정상조（218.341±22.270 pg/mL）（P＝0.000）。 I급슬관절골성관절염조환자뇨액C2C여대조조수평상차무통계학의의（ P＞0.05）。결론골성관절염환자C2C수평교정상대조조고，C2C승고수평여슬관절골성관절염병변정도평행，차이자정고도정상관。
Objective To observe the relationship between the urine C2C level and the severity of knee osteoarthritis (KOA). Methods Ninety patients with KOA were selected as KOA group , and 20 healthy volunteers were selected as control group .Five ml morning urine of each subject was collected and stored at -80℃.The anterior-posterior and lateral position of X-ray detection of knee joint was performed .The X-ray images were classified according to the Kellgren-Lawrence radiographic grading criteria .All the subjects were divided into 5 groups:control group, group I, group II, group III, and group IV, with no significant difference of age and sex.The urine C2C level was detected using enzyme-linked immunosorbent assay (ELISA).Results The urine C2C level in KOA group (218.341 ±22.270 pg/mL) was significantly higher than that in control group (261.235 ±39.944 pg/mL) ( P<0.01).The urine C2C level in group IV, group III, group II, group I, and control group was 311.872 ±18.759 pg/mL, 273.753 ±18.595 pg/mL, 256.251 ±23.379 pg/mL, 219.009 ±13.431 pg/mL, and 218.341 ±22.270 pg/mL, respectively, and the difference was significant (P=0.000).No significant difference of the urine C2C level between group I and control group was observed (P>0.05).Conclusion The urine C2C level in patients with KOA was higher than that in normal subjects .And the elevation of urine C2C level is parallel to the severity of KOA , showing a high-positive correlation.
