CAJ | 학술논문

目的：探讨补肾活血方对去势大鼠循环5-羟色胺（ Serotonin ）表达水平的影响。方法30只6月龄SD雌性大鼠去卵巢制作骨质疏松模型，另取10只行假手术，建立假手术组。3个月后，卵巢切除的大鼠随机分为三组，然后分别予补肾活血方（中药组），阿仑膦酸钠（西药组）和生理盐水（模型组）治疗，假手术组给予等量生理盐水。经过8周的治疗后处死大鼠，检测左侧股骨骨密度，ELISA检测血清Serotonin、核因子κB受体活化因子配体（ RANKL）和骨转换指标P1NP、CTX水平。结果模型组骨密度低于假手术组（ P＜0.001）；中药组和西药组全段股骨骨密度高于模型组（ P＜0.01和P＜0.001）。中药组和西药组血清P1NP低于模型组（P均＜0.001），而假手术组、中药组与西药组之间无统计学差异。中药组和西药组血清CTX低于模型组（P均＜0.001），高于假手术组（P均＜0.001）。中药组血清Serotonin水平低于西药组和模型组（P均＜0.001），假手术组与中药组之间无统计学差异。中药组和西药组血清RANKL低于模型组（ P均＜0.001），假手术组、中药组与西药组之间无统计学差异。结论补肾活血方可能是通过降低去势大鼠循环Serotonin表达水平，进而抑制RANKL表达水平，从而提高去势大鼠骨密度。
목적：탐토보신활혈방대거세대서순배5-간색알（ Serotonin ）표체수평적영향。방법30지6월령SD자성대서거란소제작골질소송모형，령취10지행가수술，건립가수술조。3개월후，란소절제적대서수궤분위삼조，연후분별여보신활혈방（중약조），아륜련산납（서약조）화생리염수（모형조）치료，가수술조급여등량생리염수。경과8주적치료후처사대서，검측좌측고골골밀도，ELISA검측혈청Serotonin、핵인자κB수체활화인자배체（ RANKL）화골전환지표P1NP、CTX수평。결과모형조골밀도저우가수술조（ P＜0.001）；중약조화서약조전단고골골밀도고우모형조（ P＜0.01화P＜0.001）。중약조화서약조혈청P1NP저우모형조（P균＜0.001），이가수술조、중약조여서약조지간무통계학차이。중약조화서약조혈청CTX저우모형조（P균＜0.001），고우가수술조（P균＜0.001）。중약조혈청Serotonin수평저우서약조화모형조（P균＜0.001），가수술조여중약조지간무통계학차이。중약조화서약조혈청RANKL저우모형조（ P균＜0.001），가수술조、중약조여서약조지간무통계학차이。결론보신활혈방가능시통과강저거세대서순배Serotonin표체수평，진이억제RANKL표체수평，종이제고거세대서골밀도。
Objective To investigate the effect of nourishing the kidney and invigorating the blood recipe on the circulating serotonin level in ovariectomized rats.Methods Thirty 6-month female Sprague Dawley rats were ovariectomized to induce osteoporosis, and another 10 rats underwent sham operation to establish sham-operated ( SHAM ) group.After 3 months, the ovariectomized rats were randomly divided into 3 groups: herb group, alendronate group, and OVX group.Rats in herb group were treated with nourishing the kidney and invigorating the blood recipe;while rats in alendronate group were treated with alendronate .Rats in OVX group and SHAM group were treated with normal saline .After 8-week treatment , all the rats were sacrificed .The bone mineral density (BMD) of the left femur was detected.The serum levels of serotonin, RANKL, and bone turnover markers, including P1NP and CTX, were detected using ELISA.Results BMD in OVX group was lower than that in SHAM group (P<0.001), while BMD in the intact femur in herb group and alendronate group was higher than that in OVX group (P<0.01 and P<0.001).The serum P1NP level in herb group and alendronate group was significantly lower than that in OVX group (P<0.001), while no significant difference among SHAM group, herb group, and alendronate group was observed.The serum CTX level in herb group and alendronate group was lower than that in OVX group (P<0.001), while it was higher than that in SHAM group (P<0.001). The serum serotonin level in herb group was significantly lower than that in OVX group and alendronate group (P<0.001).But no significant difference between SHAM group and herb group was observed.The serum RANKL level in herb group and alendronate group decreased significantly compared with that in OVX group (P<0.001), and no significant difference among SHAM group, herb group, and alendronate group was observed.Conclusion Nourishing the kidney and invigorating blood recipe can enhance BMD in OVX rats most likely by reducing the expression of serum serotonin and further by inhibiting the expression of RANKL .