CAJ | 학술논문

目的：探讨当归红芪超滤物抗辐射致乳鼠心肌细胞损伤的作用及其可能机制。方法用X线照射Wistar乳鼠原代培养的心肌细胞建立辐射损伤模型，用不同浓度的当归红芪超滤物进行干预，实验分正常对照组、辐射损伤模型组、当归红芪超滤物不同浓度（3、6、9 mg·mL-1）干预组（UFE-AH）。MTT法检测各组心肌细胞的存活率，2，4二硝基苯肼显色法检测各组细胞培养上清液中乳酸脱氢酶（ LDH）的含量，黄嘌呤氧化酶法测定各组细胞内超氧化物歧化酶（ SOD）的活性，免疫组化法观测各组细胞Bax的表达，蛋白印迹半定量测定各组细胞Bax蛋白的表达。结果与辐射损伤模型组比较，当归红芪超滤物各干预组心肌细胞存活率明显升高，LDH含量显著降低（ P﹤0.05）， SOD活性显著提高（ P﹤0.05），Bax表达显著降低（ P﹤0.05）。结论当归红芪超滤物具有拮抗辐射致心肌细胞损伤的作用，其机制与清除自由基有关。
목적：탐토당귀홍기초려물항복사치유서심기세포손상적작용급기가능궤제。방법용X선조사Wistar유서원대배양적심기세포건립복사손상모형，용불동농도적당귀홍기초려물진행간예，실험분정상대조조、복사손상모형조、당귀홍기초려물불동농도（3、6、9 mg·mL-1）간예조（UFE-AH）。MTT법검측각조심기세포적존활솔，2，4이초기분정현색법검측각조세포배양상청액중유산탈경매（ LDH）적함량，황표령양화매법측정각조세포내초양화물기화매（ SOD）적활성，면역조화법관측각조세포Bax적표체，단백인적반정량측정각조세포Bax단백적표체。결과여복사손상모형조비교，당귀홍기초려물각간예조심기세포존활솔명현승고，LDH함량현저강저（ P﹤0.05）， SOD활성현저제고（ P﹤0.05），Bax표체현저강저（ P﹤0.05）。결론당귀홍기초려물구유길항복사치심기세포손상적작용，기궤제여청제자유기유관。
Objective To explore the effect and potential mechanism on myocardial cellular damage induced by radiation in suckling mice treated with ultra - infiltration extract from Angelica Sinensis and Hedysarum Polybotrys mixture. Methods X-ray irradiation was used on the primitively cultured myocardial cells of Wistar suckling mice to set up radiation damage models. The ultra-infiltration extract from Angelica Sinensis and Hedysarum Polybotrys mixture was used for intervention at different concentrations. In the exper-iment,a normal control group,a radiation damage model group,and the intervention groups of different con-centrations(3,6,9 mg·mL-1 of the ultra-infiltration extract from Angelica Sinensis and Hedysarum Poly-botrys mixture)were divided. MTT method was used to detect the survival rate of myocardial cells in each group. The 2,4 dinitro-phenylhydrazine colorimetric reaction was used to detect LDH content in the culture supernatant. The xanthin oxidase enzyme method was used to detect SOD activity. IHC method was used to detect Bax expression and Western blotting was to detect Bax protein expression in each group. Results Compared with the radiation damage model group,the survival rate of myocardial cell was increased apparent-ly in each intervention group,LDH content was reduced significantly(P﹤0. 05),SOD activity was increased significantly(P﹤0. 05)and Bax expression was reduced significantly(P﹤0. 05). Conclusion The ultra-infiltration extract from Angelica Sinensis and Hedysarum Polybotrys mixture resists on myocardial cellular damage induced by radiation and its mechanism is related to the clearance of free radicals.