南昌大学学报（医学版）
南昌大學學報（醫學版）
남창대학학보（의학판）
ACTA ACADEMIAE MEDICINAE JIANGXI
2014年
2期
20-23,107
,共5页
磁刺激%原代神经元%transwell小室%体外%大鼠
磁刺激%原代神經元%transwell小室%體外%大鼠
자자격%원대신경원%transwell소실%체외%대서
magnetic stimulation%primary neuron%transwell chamber%in vitro%rats
目的：体外观察磁刺激对原代神经元迁移的影响。方法原代培养 SD 大鼠皮质神经元，分为正常组（con-trol，C 组）、假刺激组（shame，S 组）、40％最大输出强度组（40％ of maximum intensity of stimulation，0．4T 组）、60％最大输出强度组（60％ of maximum intensity of stimulation，0．6T 组）。各组细胞接种于 transwell 小室内，自细胞接种后第2～6天接受磁刺激，连续刺激5 d，各组细胞于第6天同一时间染色，将 transwell 小室倒置，光学显微镜下计数。结果C 组有（2．6±0．548）个、S 组有（3．0±0．707）个细胞迁移，2组比较差异无统计学意义（P ＞0．05）；0．4T 组有（17．40±1．673）个、0．6T 组有（18．40±1．572）个细胞迁移，迁移的数量较 C 组及 S 组明显增多（P ＜0．05）。结论磁刺激可促进体外原代神经元迁移。
目的：體外觀察磁刺激對原代神經元遷移的影響。方法原代培養 SD 大鼠皮質神經元，分為正常組（con-trol，C 組）、假刺激組（shame，S 組）、40％最大輸齣彊度組（40％ of maximum intensity of stimulation，0．4T 組）、60％最大輸齣彊度組（60％ of maximum intensity of stimulation，0．6T 組）。各組細胞接種于 transwell 小室內，自細胞接種後第2～6天接受磁刺激，連續刺激5 d，各組細胞于第6天同一時間染色，將 transwell 小室倒置，光學顯微鏡下計數。結果C 組有（2．6±0．548）箇、S 組有（3．0±0．707）箇細胞遷移，2組比較差異無統計學意義（P ＞0．05）；0．4T 組有（17．40±1．673）箇、0．6T 組有（18．40±1．572）箇細胞遷移，遷移的數量較 C 組及 S 組明顯增多（P ＜0．05）。結論磁刺激可促進體外原代神經元遷移。
목적：체외관찰자자격대원대신경원천이적영향。방법원대배양 SD 대서피질신경원，분위정상조（con-trol，C 조）、가자격조（shame，S 조）、40％최대수출강도조（40％ of maximum intensity of stimulation，0．4T 조）、60％최대수출강도조（60％ of maximum intensity of stimulation，0．6T 조）。각조세포접충우 transwell 소실내，자세포접충후제2～6천접수자자격，련속자격5 d，각조세포우제6천동일시간염색，장 transwell 소실도치，광학현미경하계수。결과C 조유（2．6±0．548）개、S 조유（3．0±0．707）개세포천이，2조비교차이무통계학의의（P ＞0．05）；0．4T 조유（17．40±1．673）개、0．6T 조유（18．40±1．572）개세포천이，천이적수량교 C 조급 S 조명현증다（P ＜0．05）。결론자자격가촉진체외원대신경원천이。
Objective To observe the effects of magnetic stimulation on primary neuronal mi-gration in vitro.Methods Primary cultured SD rat cortical neurons were divided into four group:control group(group C),sham simulation group(group S),40% of maximum stimulation intensity group(0.4 T group),and 60% of maximum stimulation intensity group(0.6 T group).Cells were planted in the transwell chambers,and were stimulated from the second day to the sixth day after plantation.Moreover,cells in different groups were stained at the same time on the sixth day.Tr-answell chambers were inverted and cells were counted under light microscope.Results The numbers of migrated cells in 0.4T group(17.40±1.673)and 0.6T group(18.40±1.572)were significantly higher than those in group C(2.6±0.548)or group S(3.0±0.707)(P <0.05).The differences were not significant between group C and group S(P >0.05).Conclusion Magnetic stimulation can promote primary neuronal migration in vitro.
