CAJ | 학술논문

　　目的探讨HIV-1 Nef蛋白能否通过调控PTEN/PI3K信号通路促进卡波氏肉瘤病毒（KSHV） vIL-6诱导血管生成。方法用脂质体转染的方法将pPTEN-cDNA、PI3K-DN及其对照空载体质粒分别转染稳定表达KSHV vIL-6和HIV-1 Nef蛋白的内皮细胞，采用微管形成试验观察微管形成状况，将这些细胞接种鸡胚绒毛尿囊膜（CAM）检测血管生成情况。 Western blot进一步检测转染了上述质粒后细胞内源性PTEN和PI3K信号分子的表达水平。结果过表达PTEN或抑制PI3K表达均可抑制Nef促进vIL-6诱导的内皮细胞微管形成和CAM血管生成。结论 HIV-1 Nef蛋白通过调控PTEN/PI3K信号通路促进KSHV vIL-6诱导血管生成。
　　목적탐토HIV-1 Nef단백능부통과조공PTEN/PI3K신호통로촉진잡파씨육류병독（KSHV） vIL-6유도혈관생성。방법용지질체전염적방법장pPTEN-cDNA、PI3K-DN급기대조공재체질립분별전염은정표체KSHV vIL-6화HIV-1 Nef단백적내피세포，채용미관형성시험관찰미관형성상황，장저사세포접충계배융모뇨낭막（CAM）검측혈관생성정황。 Western blot진일보검측전염료상술질립후세포내원성PTEN화PI3K신호분자적표체수평。결과과표체PTEN혹억제PI3K표체균가억제Nef촉진vIL-6유도적내피세포미관형성화CAM혈관생성。결론 HIV-1 Nef단백통과조공PTEN/PI3K신호통로촉진KSHV vIL-6유도혈관생성。
Objective To investigate whether HIV-1 Nef could promote the angiogenesis and tu-morigenesis induced by KSHV vIL-6 through regulating PTEN/PI3K signaling pathway .Methods Lipo-some transfection was used to transfect cDNA of pPTEN , dominant-negative ( DN) construct of PI3K and control vector into endothelial cells , which stably express KSHV vIL-6 and HIV-1 Nef.Microtubule forma-tion assay and chicken chorioallantoic membrane ( CAM) assay were used to evaluate microtubule formation and angiogenesis , respectively .Expressions of PTEN and PI 3 K were measured by Western blot .Results Both overexpression of PTEN and inhibited expression of PI 3K suppressed the vIL-6-induced microtubule for-mation and angiogenesis in CAM mediated by Nef .Conclusion HIV-1 Nef enhances vIL-6-induced angio-genesis and tumorigenesis through regulating PTEN/PI3K signaling pathway .