中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
8期
574-577
,共4页
惠长野%刘晓露%郭妍%彭亮%张艳芳%王佃鹏%曹虹
惠長野%劉曉露%郭妍%彭亮%張豔芳%王佃鵬%曹虹
혜장야%류효로%곽연%팽량%장염방%왕전붕%조홍
脑膜炎大肠埃希菌%OmpT%脑微血管内皮细胞%黏附%毒力
腦膜炎大腸埃希菌%OmpT%腦微血管內皮細胞%黏附%毒力
뇌막염대장애희균%OmpT%뇌미혈관내피세포%점부%독력
Meningitic Escherichia coli%OmpT%Brain microvascular endothelial cell%Adhesion%Virulence
目的探讨新生儿脑膜炎大肠埃希菌ompT对菌株毒力的影响。方法对比野生致病株E44及ompT敲除株E44∶ΔompT两者体外黏附人脑微血管内皮细胞的能力；构建回复株，并考察ompT回补质粒pST及点突变质粒pST85能否恢复敲除株的黏附能力；考察ompT缺失对菌株定植乳鼠肠道能力的影响；考察 ompT 缺失对菌株穿越乳鼠血脑屏障的影响。结果相比野生株， E44∶ΔompT黏附人脑微血管内皮细胞能力明显降低；低拷贝质粒pST及点突变质粒pST85部分恢复了E44∶ΔompT的黏附能力；ompT缺失不影响菌株肠道定植能力；E44∶ΔompT可诱导与野生株相似水平的菌血症，但穿越乳鼠血脑屏障的能力显著降低。结论 ompT与菌体黏附脑微血管内皮细胞及穿越血脑屏障相关，OmpT蛋白水解酶活性在其中的作用有待进一步研究。
目的探討新生兒腦膜炎大腸埃希菌ompT對菌株毒力的影響。方法對比野生緻病株E44及ompT敲除株E44∶ΔompT兩者體外黏附人腦微血管內皮細胞的能力；構建迴複株，併攷察ompT迴補質粒pST及點突變質粒pST85能否恢複敲除株的黏附能力；攷察ompT缺失對菌株定植乳鼠腸道能力的影響；攷察 ompT 缺失對菌株穿越乳鼠血腦屏障的影響。結果相比野生株， E44∶ΔompT黏附人腦微血管內皮細胞能力明顯降低；低拷貝質粒pST及點突變質粒pST85部分恢複瞭E44∶ΔompT的黏附能力；ompT缺失不影響菌株腸道定植能力；E44∶ΔompT可誘導與野生株相似水平的菌血癥，但穿越乳鼠血腦屏障的能力顯著降低。結論 ompT與菌體黏附腦微血管內皮細胞及穿越血腦屏障相關，OmpT蛋白水解酶活性在其中的作用有待進一步研究。
목적탐토신생인뇌막염대장애희균ompT대균주독력적영향。방법대비야생치병주E44급ompT고제주E44∶ΔompT량자체외점부인뇌미혈관내피세포적능력；구건회복주，병고찰ompT회보질립pST급점돌변질립pST85능부회복고제주적점부능력；고찰ompT결실대균주정식유서장도능력적영향；고찰 ompT 결실대균주천월유서혈뇌병장적영향。결과상비야생주， E44∶ΔompT점부인뇌미혈관내피세포능력명현강저；저고패질립pST급점돌변질립pST85부분회복료E44∶ΔompT적점부능력；ompT결실불영향균주장도정식능력；E44∶ΔompT가유도여야생주상사수평적균혈증，단천월유서혈뇌병장적능력현저강저。결론 ompT여균체점부뇌미혈관내피세포급천월혈뇌병장상관，OmpT단백수해매활성재기중적작용유대진일보연구。
Objective To investigate the virulence role of ompT of Escherichia coli in the patho-genesis of neonatal meningitis .Methods Adhesive abilities of the parent strain E 44 and the isogenic ompT-deletion mutant strain ( E44 ∶ΔompT) to human brain microvascular endothelial cells were evaluated in in vitro model.Low-copy-number plasmid pST containing ompT locus and point mutant plasmid pST 85 were transferred into E44 ∶ΔompT to construct the complemented mutant strain , and its adhesive ability was ana-lyzed.Influences of ompT deletion on E44 strain in its ability of bacterial intestinal colonization and ability of penetrating the blood-brain barrier were determined . Results In comparison with the parent strain , E44 ∶ΔompT strain showed significantly impaired adhesive ability to human brain microvascular endothelial cells, which could be partly restored by inserting the complementary plasmids of pST and pST 85.Deletion of the ompT did not affect Escherichia coli K1 in normal intestinal colonization in in vivo model.E44 ∶ΔompT strain could induce bacteremia , which was similar to that induced by the parent strain , but its ability of crossing the blood-brain barrier was significantly declined .Conclusion The study demonstrate that ompT plays an important role as the virulence element of Escherichia coli in binding to brain microvascular endothe-lial cells and penetrating the blood-brain barrier .Further study should be performed to investigate the influ-ences of OmpT proteinase on the virulence of Escherichia coil.
