CAJ | 학술논문

　　目的探讨二甲双胍对子宫内膜癌细胞系Ishikawa细胞增殖、凋亡和细胞周期的影响。方法以不同浓度及时间二甲双胍作用Ishikawa细胞，采用四甲基偶氮唑蓝比色法和流式细胞仪检测细胞增殖、凋亡和细胞周期。结果二甲双胍抑制子宫内膜癌细胞增殖，并与作用时间浓度相关。流式细胞仪检测提示二甲双胍作用下细胞中G0/G1期细胞比例增加，S期细胞比例降低。结论二甲双胍能抑制子宫内膜癌Ishika-wa细胞增殖，使细胞生长周期停滞。
　　목적탐토이갑쌍고대자궁내막암세포계Ishikawa세포증식、조망화세포주기적영향。방법이불동농도급시간이갑쌍고작용Ishikawa세포，채용사갑기우담서람비색법화류식세포의검측세포증식、조망화세포주기。결과이갑쌍고억제자궁내막암세포증식，병여작용시간농도상관。류식세포의검측제시이갑쌍고작용하세포중G0/G1기세포비례증가，S기세포비례강저。결론이갑쌍고능억제자궁내막암Ishika-wa세포증식，사세포생장주기정체。
Objective To investigate the effect of metformin on proliferation , cell cycle distribution and ap-optosis of human endometrial carcinoma cell lines in vitro .Methods The effects of metformin on proliferation , cell cycle distribution and apoptosis of Ishikawa cell lines were measured by MTT assay and fluorescence -activated cell sorting technique .Results Metformin potently inhibited growth in a dose-dependent manner in Ishikawa cell lines in a dose-and time-dependent fashion .Flow cytometric analysis showed that metformin increased the number of cells in G0/G1 and reduced the percentage of cells in S phase .Conclusion Metformin is a potent inhibitor of cell prolifera-tion in endometrial cancer cell lines .