CAJ | 학술논문

　　目的探讨联合测定胸水中TOLL样受体2（TLR2）和γ-干扰素（IFN-γ）水平对结核性胸腔积液的诊断价值。方法收集确诊结核性胸腔积液、恶性胸腔积液和细菌性胸腔积液患者的胸水和血清，应用酶联免疫吸附试剂盒（ELISA）测定TLR2和IFN-γ的浓度，并对数据进行分析。结果（1）结核组患者胸水TLR2（22.19±2.12） ng/L、IFN-γ（2287.20±408.34） ng/L均比其相应血清水平高，差异有统计学意义（ P＜0.05）。结核组患者胸水的TLR2和IFN-γ水平均高于恶性组和细菌组，差异有统计学意义（ P＜0.05）。（2） TLR2诊断结核性胸腔积液的工作特征曲线（ ROC）下面积（ ACU）为0.696（95％CI：0.501～0.849），灵敏度为66.67％，特异度为80％。同样，IFN-γ的ACU为0.945（95％CI：0.875～1.014），灵敏度为87.5％，特异度为97.9％。结论联合测定TLR2和IFN-γ指标有助于结核性胸腔积液的诊断。
　　목적탐토연합측정흉수중TOLL양수체2（TLR2）화γ-간우소（IFN-γ）수평대결핵성흉강적액적진단개치。방법수집학진결핵성흉강적액、악성흉강적액화세균성흉강적액환자적흉수화혈청，응용매련면역흡부시제합（ELISA）측정TLR2화IFN-γ적농도，병대수거진행분석。결과（1）결핵조환자흉수TLR2（22.19±2.12） ng/L、IFN-γ（2287.20±408.34） ng/L균비기상응혈청수평고，차이유통계학의의（ P＜0.05）。결핵조환자흉수적TLR2화IFN-γ수평균고우악성조화세균조，차이유통계학의의（ P＜0.05）。（2） TLR2진단결핵성흉강적액적공작특정곡선（ ROC）하면적（ ACU）위0.696（95％CI：0.501～0.849），령민도위66.67％，특이도위80％。동양，IFN-γ적ACU위0.945（95％CI：0.875～1.014），령민도위87.5％，특이도위97.9％。결론연합측정TLR2화IFN-γ지표유조우결핵성흉강적액적진단。
Objective To explore the value of the combined detection of Toll-like receptor-2(TLR2) and in-terferon-γ( IFN-γ) in pleural effusion in the diagnosis of tuberculous pleural effusion .Methods The concentrations of soluble TLR2 and IFN-γin pleural effusion and serum were determined by enzyme linked immunosorbent assay (ELISA) in patients with tuberculous, malignant and bacterial pleural effusion.Results (1)The concentrations of sTLR2[22.19 ±2.12)ng/L] and IFN-γ[(2 287.20 ±408.34)ng/L] in pleural effusions were significantly higher than those in serum of the same patients .The levels of sTLR2 and IFN-γin tuberculous pleural effusion was signifi-cantly higher than those in malignant and in bacterial pleural effusion .(2) The ROC analysis for TLR2 and IFN-γwere done.The levels of TLR2 generated an area under the receive operating characteristic (ROC) curve of 0.696 [95%confidence interval (CI):0.501~0.849], at a sensitivity of 66.67%, a specificity of 80%.As well as IFN-γgenerated an area under the ROC curve of 0.945(95%CI:0.875~1.014), at a sensitivity of 87.5%, a specificity of 97.9 %.Conclusion The combined decection of TLR 2 and IFN-γin pleural effusion may be helpful in the differ-ential diagnosis of tuberculous pleural effusion and the other pleural effusion , which provides an important information for clinical diagnosis and treatment .