中国动物保健
中國動物保健
중국동물보건
CHINA ANIMAL HEALTH
2013年
8期
30-32
,共3页
高效液相色谱法%磺胺间甲氧嘧啶%甲氧苄啶%复方磺胺间甲氧嘧啶钠可溶性粉
高效液相色譜法%磺胺間甲氧嘧啶%甲氧芐啶%複方磺胺間甲氧嘧啶鈉可溶性粉
고효액상색보법%광알간갑양밀정%갑양변정%복방광알간갑양밀정납가용성분
HPLC%sulfamonomethoxine%TMP%compound sulfamonomethoxine sodium soluble powder
建立复方磺胺间甲氧嘧啶钠可溶性粉中磺胺间甲氧嘧啶钠(以磺胺间甲氧嘧啶计)和甲氧苄啶含量的HPLC检测方法,采用Inertsil C18色谱柱(4.6mm×250mm,5μm),以乙腈:0.017mol/L磷酸液(20:80)(按1‰的比例加入三乙胺)为流动相,流速为1mL/min,检测波长为230nm。在该色谱条件下,两种药物分离很好,磺胺间甲氧嘧啶在24.89~248.9μg/mL之间,甲氧苄啶在5~50μg/mL之间,线性良好(r>0.99999),添加回收率在97.46%~100.45%之间。该方法快速简便,准确可靠,能够满足实际样品的检测要求。
建立複方磺胺間甲氧嘧啶鈉可溶性粉中磺胺間甲氧嘧啶鈉(以磺胺間甲氧嘧啶計)和甲氧芐啶含量的HPLC檢測方法,採用Inertsil C18色譜柱(4.6mm×250mm,5μm),以乙腈:0.017mol/L燐痠液(20:80)(按1‰的比例加入三乙胺)為流動相,流速為1mL/min,檢測波長為230nm。在該色譜條件下,兩種藥物分離很好,磺胺間甲氧嘧啶在24.89~248.9μg/mL之間,甲氧芐啶在5~50μg/mL之間,線性良好(r>0.99999),添加迴收率在97.46%~100.45%之間。該方法快速簡便,準確可靠,能夠滿足實際樣品的檢測要求。
건립복방광알간갑양밀정납가용성분중광알간갑양밀정납(이광알간갑양밀정계)화갑양변정함량적HPLC검측방법,채용Inertsil C18색보주(4.6mm×250mm,5μm),이을정:0.017mol/L린산액(20:80)(안1‰적비례가입삼을알)위류동상,류속위1mL/min,검측파장위230nm。재해색보조건하,량충약물분리흔호,광알간갑양밀정재24.89~248.9μg/mL지간,갑양변정재5~50μg/mL지간,선성량호(r>0.99999),첨가회수솔재97.46%~100.45%지간。해방법쾌속간편,준학가고,능구만족실제양품적검측요구。
To establish an HPLC method for determination of sulfamonomethoxine and TMP in Compound sulfa-monomethoxine sodium soluble powder. Sulfamonomethoxine and TMP in the soluble powder were determined by HPLC with a Inertsil C18 column (4.6mm×250mm,5μm)with UV detection at 230nm, and the mobile phase was consisted of acetonitrile-0.017mol/L phosphoric acid (20:80,)(the proportion of 1‰to join triethylamine). The flow rate was 1.0ml/min. Sulfamonomethoxine and TMP in compound sulfamonomethoxine sodium soluble powder can be separated well under the chromatographic condition. The calibration curve was linear (r>0.99999)within the range of 24.89~248.9μg/mL for sulfamonomethoxine. It the range of 5~50μg/mL for TMP, the recovery was 97.46%~100.45%.The method is simple, quick, accurate and can be meet the detection requirements.