CAJ | 학술논문

　　目的研究丁基苯酞对弥漫性脑创伤大鼠运动功能及学习记忆能力的影响。方法128只雄性Sprague-Dawley大鼠随机分为假手术组、创伤组、丁基苯酞低剂量组和丁基苯酞高剂量组，每组各32只。假手术组不致伤，丁基苯酞低、高剂量组分别注射丁基苯酞80 mg/kg和160 mg/kg，每24小时注射1次。采用Marmarou法建立大鼠弥漫性颅脑损伤模型。术后24 h、48 h、72 h取大脑皮层冠状缝左右0.2 cm组织，光镜和电镜下观察脑组织超微结构变化，对大鼠综合运动能力以及学习记忆功能进行评测。结果与创伤组比较，丁基苯酞低、高剂量组大鼠脑组织超微结构，包括神经元内细胞器、轴索及毛细血管等超微结构的损伤程度减轻，计数存活神经元密度明显增加(P<0.01)；大鼠逃避潜伏期时间明显缩短(P<0.01)，72 h亚组穿越平台次数明显增多(P<0.01)；综合运动能力评分提高(P<0.05)。上述指标变化在高剂量组更为显著。结论丁基苯酞可改善弥漫性脑创伤后运动和学习记忆能力损伤，其机制与减少神经元丢失，减轻脑组织超微结构损害有关。
　　목적연구정기분태대미만성뇌창상대서운동공능급학습기억능력적영향。방법128지웅성Sprague-Dawley대서수궤분위가수술조、창상조、정기분태저제량조화정기분태고제량조，매조각32지。가수술조불치상，정기분태저、고제량조분별주사정기분태80 mg/kg화160 mg/kg，매24소시주사1차。채용Marmarou법건립대서미만성로뇌손상모형。술후24 h、48 h、72 h취대뇌피층관상봉좌우0.2 cm조직，광경화전경하관찰뇌조직초미결구변화，대대서종합운동능력이급학습기억공능진행평측。결과여창상조비교，정기분태저、고제량조대서뇌조직초미결구，포괄신경원내세포기、축색급모세혈관등초미결구적손상정도감경，계수존활신경원밀도명현증가(P<0.01)；대서도피잠복기시간명현축단(P<0.01)，72 h아조천월평태차수명현증다(P<0.01)；종합운동능력평분제고(P<0.05)。상술지표변화재고제량조경위현저。결론정기분태가개선미만성뇌창상후운동화학습기억능력손상，기궤제여감소신경원주실，감경뇌조직초미결구손해유관。
Objective To study the effects of 3-n-butylphthalide (NBP) on motor function and learning and memory ability in rats with diffuse brain injury (DBI). Methods 128 male Sprague-Dawley rats were randomly divided into sham operation group, traumatic group, low-dose NBP treatment group and high-dose NBP treatment group with 32 rats in each group. Every group was divided into 24 h, 48 h and 72 h subgroups. DBI rat model was established according to the description of Marmarou's diffused brain injury. The histopathologic chang-es of cerebral tissue (0.2 cm bilateralis coronal line) were observed by light and electron microscope at 24 h, 48 h and 72 h after injury. Mor-ris water maze and rolling test were performed daily at 24, 48 and 72 h (time points). Results Compared with the model group, the damage of brain tissue decreased, and the survival nerve cells increased (P<0.01). The behavioral tests showed that the latency to find the platform shortened (P<0.01) and the frequency of crossing the platform increased in the 72 h subgroup (P<0.01). The general movement ability en-hanced in NBP groups (P<0.05). All the indexes were more significant in high-dose NBP treatment group. Conclusion NBP can improve neurological function and learning and memory function after brain injury and the molecular mechanisms is related to the decrease of the nerve cells lose and traumatic cerebral ultrastructure damage.