CAJ | 학술논문

　　探讨了木聚糖酶-纤维素结构结合结构域（ CBM）基因融合过程，在不加引物而只用两种DNA片段经PCR扩增全长融合基因，从而证明了自我延伸过程的存在，是指互补匹配的两段DNA互为引物、互为模板在聚合酶作用下合成融合基因的过程，从理论上阐明了PCR中的自我延伸过程。在传统PCR中加入3次循环的自我延伸程序（94℃×1 min，52℃×1 min，72℃×1 min），融合基因扩增量要更多。进而探讨自我延伸程序中延伸时间的影响，发现延伸时间2、3、5 min扩增的融合基因量显著增强；而延伸时间为1 min时，融合基因条带的亮度增加不明显，说明延伸时间是增加扩增量的关键因素。
　　탐토료목취당매-섬유소결구결합결구역（ CBM）기인융합과정，재불가인물이지용량충DNA편단경PCR확증전장융합기인，종이증명료자아연신과정적존재，시지호보필배적량단DNA호위인물、호위모판재취합매작용하합성융합기인적과정，종이론상천명료PCR중적자아연신과정。재전통PCR중가입3차순배적자아연신정서（94℃×1 min，52℃×1 min，72℃×1 min），융합기인확증량요경다。진이탐토자아연신정서중연신시간적영향，발현연신시간2、3、5 min확증적융합기인량현저증강；이연신시간위1 min시，융합기인조대적량도증가불명현，설명연신시간시증가확증량적관건인소。
This article explored the fusion process of xylanase and Cellulose -binding Module ( CBM) , and amplified the com-plete fusion gene by PCR under the conditions of no using primers and only using the gene fragments of xylanase and CBM, which proved the existence of self -extension process .Self-extension process was the synthetic process of the fusion gene under the action of polymerase and two complementary DNA fragments ( mutual primers and mutual templates ) , and the self-extension PCR process was clarified theoretically .Thus, adding 3 cycles of self-extension program (94℃for 1 min, 52℃for 1 min, 72 ℃for 1 min) in the traditional PCR could obtain more fusion gene amplification amount .The influences of extended time on the fusion gene am-plification amount were studied , and it was found that the fusion gene amplification amount was significantly increased when the ex -tended time was 2, 3, 5 min;the brightness increment of fusion gene band was not obvious as the extended time was 1 min, which indicated that extension time was a key factor increasing the amount of amplification .