海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
5期
38-40
,共3页
绿原酸%滨蒿内酯%复方胆通胶囊%HPLC
綠原痠%濱蒿內酯%複方膽通膠囊%HPLC
록원산%빈호내지%복방담통효낭%HPLC
Chlorogenic acid%Scoparone%compound Dantong Capsules%HPLC
目的：建立测定复方胆通胶囊中绿原酸和滨蒿内酯的含量的高效液相色谱法。方法采用高效液相色谱法，Agilent Zorbax Extend-C18色谱柱（4.6mm ×250mm，5μm）44nm。结果绿原酸和滨蒿内酯分别在0.9168～9.1678μg· mL -1、0.4960～4.9600μg· mL -1范围内呈良好的线性关系（均为r＝0.9999），平均回收率分别为99.12％、98.92％（n＝9），RSD＝2.0％、RSD＝2.1％。结论本方法操作简便，结果准确、可靠。
目的：建立測定複方膽通膠囊中綠原痠和濱蒿內酯的含量的高效液相色譜法。方法採用高效液相色譜法，Agilent Zorbax Extend-C18色譜柱（4.6mm ×250mm，5μm）44nm。結果綠原痠和濱蒿內酯分彆在0.9168～9.1678μg· mL -1、0.4960～4.9600μg· mL -1範圍內呈良好的線性關繫（均為r＝0.9999），平均迴收率分彆為99.12％、98.92％（n＝9），RSD＝2.0％、RSD＝2.1％。結論本方法操作簡便，結果準確、可靠。
목적：건립측정복방담통효낭중록원산화빈호내지적함량적고효액상색보법。방법채용고효액상색보법，Agilent Zorbax Extend-C18색보주（4.6mm ×250mm，5μm）44nm。결과록원산화빈호내지분별재0.9168～9.1678μg· mL -1、0.4960～4.9600μg· mL -1범위내정량호적선성관계（균위r＝0.9999），평균회수솔분별위99.12％、98.92％（n＝9），RSD＝2.0％、RSD＝2.1％。결론본방법조작간편，결과준학、가고。
OBJECTIVE To establish a method for the determination of chlorogenic acid and scoparone in com-pound Dantong Capsules by HPLC.METHODS A Zobax Extend-C18 column was used.The mobile phase was Ace-tonitrile-0.05% phosphoric acid with gradient elution analysis and the detection wavelength was 327 nm and 345nm.RESUTLS The linear range for Chlorogenic acid and Scoparone was 0.9168~9.1678μg· mL-1、0.4960~4.9600μg · mL-1、( r =0.9999 ) , the average recovery was 99.12%、98.92%( n =9 ) and RSD was 2.0%、2.1%,respectively.CONCLUSION The method used for the determination of chlorogenic acid and scoparone in compound Dantong capsules is simple ,timesaving and accurate.
