海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
5期
32-34
,共3页
微丸%HPLC法%丹酚酸B
微汍%HPLC法%丹酚痠B
미환%HPLC법%단분산B
Micro pill%HPLC%Salvianolic acid B
目的：用高效液相色谱法测定复方丹参微丸中丹酚酸B的含量。方法以乙腈-甲醇-甲酸-水（10∶30∶1∶59）为流动相，色谱柱 Diamon-silTM C18（5μm，4.6×250mm），波长286nm、柱温40℃、流速1.0mL· min -1为色谱条件，对复方丹参微丸中丹酚酸 B含量进行分析。结果丹酚酸B在59.5～1487.5 ng范围内成良好的线性关系（ r＝0.99995）。结论本法简便、准确、重现性好。
目的：用高效液相色譜法測定複方丹參微汍中丹酚痠B的含量。方法以乙腈-甲醇-甲痠-水（10∶30∶1∶59）為流動相，色譜柱 Diamon-silTM C18（5μm，4.6×250mm），波長286nm、柱溫40℃、流速1.0mL· min -1為色譜條件，對複方丹參微汍中丹酚痠 B含量進行分析。結果丹酚痠B在59.5～1487.5 ng範圍內成良好的線性關繫（ r＝0.99995）。結論本法簡便、準確、重現性好。
목적：용고효액상색보법측정복방단삼미환중단분산B적함량。방법이을정-갑순-갑산-수（10∶30∶1∶59）위류동상，색보주 Diamon-silTM C18（5μm，4.6×250mm），파장286nm、주온40℃、류속1.0mL· min -1위색보조건，대복방단삼미환중단분산 B함량진행분석。결과단분산B재59.5～1487.5 ng범위내성량호적선성관계（ r＝0.99995）。결론본법간편、준학、중현성호。
OBJECTIVE To set up a method for the determination of content of Salvianolic acid B in compound Salvia Miltiorrhiza Micro Pills by HPLC.MEHTODS With acetonitrile-methanol-formic acid and water (10∶30∶1∶59) as the mobile phase for elution,the chromatographic conditions were as follows:DiamonsilTM C18 column (5μm, 4.6 ×250mm),the detecting wavelength of 286 nm,the flowing rate was 1.0mL· min-1 with the column tempera-ture at 40 ℃,the of content Salvianolic acid B in the compound salvia miltiorrhiza Micro Pills analyzed.RESLUTS Dan phenolic acids content of B showed a good linear relationship at the range of 59.5~1487.5 ng(r=0.99995).CONCLUSION This method has a good reproducibility ,quantitative ,accurate and can be used as the preparation method of quantitative analysis.
