海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
5期
24-25
,共2页
HPLC%丹芍康妇丸%人参皂苷Rg1
HPLC%丹芍康婦汍%人參皂苷Rg1
HPLC%단작강부환%인삼조감Rg1
HPLC%Danshaokangfu Pills%Ginsenoside Rg 1
目的：建立HPLC法测定丹芍康妇丸中人参皂苷Rg1的含量。方法采用HPLC 法，以十八烷基硅烷键合硅胶为填充剂；以乙腈-水为流动相。以乙腈（A）∶水（B）按0～20min A∶B为19％∶81％梯度到40％∶60％；20～25min为40％∶60％进行洗脱；检测波长203nm。理论板数按人参皂苷Rg1计算应不低于5000。结果人参皂苷Rg1对照品浓度在0.495～15.84μg之间与其峰面积呈良好的线性关系，r＝0.9998。加样平均回收率为98.0％，RSD＝1.6％。结论本方法简单，准确，重现性好，可用于丹芍康妇丸中三七含量的测定。
目的：建立HPLC法測定丹芍康婦汍中人參皂苷Rg1的含量。方法採用HPLC 法，以十八烷基硅烷鍵閤硅膠為填充劑；以乙腈-水為流動相。以乙腈（A）∶水（B）按0～20min A∶B為19％∶81％梯度到40％∶60％；20～25min為40％∶60％進行洗脫；檢測波長203nm。理論闆數按人參皂苷Rg1計算應不低于5000。結果人參皂苷Rg1對照品濃度在0.495～15.84μg之間與其峰麵積呈良好的線性關繫，r＝0.9998。加樣平均迴收率為98.0％，RSD＝1.6％。結論本方法簡單，準確，重現性好，可用于丹芍康婦汍中三七含量的測定。
목적：건립HPLC법측정단작강부환중인삼조감Rg1적함량。방법채용HPLC 법，이십팔완기규완건합규효위전충제；이을정-수위류동상。이을정（A）∶수（B）안0～20min A∶B위19％∶81％제도도40％∶60％；20～25min위40％∶60％진행세탈；검측파장203nm。이론판수안인삼조감Rg1계산응불저우5000。결과인삼조감Rg1대조품농도재0.495～15.84μg지간여기봉면적정량호적선성관계，r＝0.9998。가양평균회수솔위98.0％，RSD＝1.6％。결론본방법간단，준학，중현성호，가용우단작강부환중삼칠함량적측정。
OBJECTIVE To establish a HPLC method for determine ginsenoside Rg 1 in Danshaokangfu Pills.METHODS The HPLC was applied with the condition as follows:column:Merck C18(5μm,150 ×4.6mm), mobile phase:ACN( A)∶water( B) by 0~20 minute A∶B was 19%∶81%to 40%∶60%;20~25 gradient elution was 40%∶60% minutes;( 19∶81 ).flow speed:1.0 mL · min-1 , the column temperature was 20℃, detection:203 nm.RESULTS The linear range of ginsenoside Rg 1 was 0.495~15.84μg r=0.9998.The average recovery of ginsenoside Rg 1 was 98.0%,RSD=1.6%.CONCLUSION The method is simple ,accurate and specific ,it can be used for quality control of Danshaokangfu Pills.
