广西医学
廣西醫學
엄서의학
GUANGXI MEDICAL JOURNAL
2014年
5期
624-626
,共3页
陈小婷%欧斌贤%唐屈%黄积荣%刘达恩%农庆文
陳小婷%歐斌賢%唐屈%黃積榮%劉達恩%農慶文
진소정%구빈현%당굴%황적영%류체은%농경문
增生性瘢痕%成纤维细胞%苦参碱%基质金属蛋白酶
增生性瘢痕%成纖維細胞%苦參堿%基質金屬蛋白酶
증생성반흔%성섬유세포%고삼감%기질금속단백매
Hyperplastic scar%Fibroblasts%Matrine%Matrix metalloproteinase
目的:探讨苦参碱对体外培养人增生性瘢痕成纤维细胞( HSFb )基质金属蛋白酶( MMP-1、MMP-9)表达的影响。方法将浓度分别为0 mmol/L(对照组)、2.5 mmol/L、5.0 mmol/L、10.0 mmol/L苦参碱作用于体外培养人HSFb,采用酶联免疫吸附试验检测加药后上清液中MMP-1、MMP-9表达量。结果苦参碱作用24 h、48 h时各浓度组MMP-1、MMP-9的表达量均高于对照组(P<0.05),5.0 mmol/L组表达量明显高于2.5 mmol/L组、10.0 mmol/L组( P<0.05)。结论苦参碱可增加HSFb MMP-1、MMP-9的表达,抗瘢痕作用好,在增生性瘢痕防治上具有一定的应用前景。
目的:探討苦參堿對體外培養人增生性瘢痕成纖維細胞( HSFb )基質金屬蛋白酶( MMP-1、MMP-9)錶達的影響。方法將濃度分彆為0 mmol/L(對照組)、2.5 mmol/L、5.0 mmol/L、10.0 mmol/L苦參堿作用于體外培養人HSFb,採用酶聯免疫吸附試驗檢測加藥後上清液中MMP-1、MMP-9錶達量。結果苦參堿作用24 h、48 h時各濃度組MMP-1、MMP-9的錶達量均高于對照組(P<0.05),5.0 mmol/L組錶達量明顯高于2.5 mmol/L組、10.0 mmol/L組( P<0.05)。結論苦參堿可增加HSFb MMP-1、MMP-9的錶達,抗瘢痕作用好,在增生性瘢痕防治上具有一定的應用前景。
목적:탐토고삼감대체외배양인증생성반흔성섬유세포( HSFb )기질금속단백매( MMP-1、MMP-9)표체적영향。방법장농도분별위0 mmol/L(대조조)、2.5 mmol/L、5.0 mmol/L、10.0 mmol/L고삼감작용우체외배양인HSFb,채용매련면역흡부시험검측가약후상청액중MMP-1、MMP-9표체량。결과고삼감작용24 h、48 h시각농도조MMP-1、MMP-9적표체량균고우대조조(P<0.05),5.0 mmol/L조표체량명현고우2.5 mmol/L조、10.0 mmol/L조( P<0.05)。결론고삼감가증가HSFb MMP-1、MMP-9적표체,항반흔작용호,재증생성반흔방치상구유일정적응용전경。
Objective To study the effects of matrine on the expressions of matrix metalloproteinase (MMP)-1, MMP-9 in human hypertropic scar fibroblasts ( HSFb) in vitro.Methods HSFb was cultured in vitro by being treated with different concentrations of matrine[0 mmol/L(control group),2.5 mmol/L,5.0 mmol/L,10.0 mmol/L].Enzyme-linked immunoadsorbent assay(ELISA) was used to detect the expressions of MMP-1,MMP-9 in the treated supernatants.Results The expression levels of MMP-1,MMP-9 in groups of different concentrations of matrine were higher than those in the control group 24 or 48 hours after matrine treatment(P<0.05),the expression levels of MMP-1,MMP-9 in the 5.0 mmol/L matrine group were higher than those in the 2.5,10.0 mmol/L matrine groups ( P<0.05).Conclusion Matrine can increase the expression of MMP-1,MMP-9.It can be applied to hyperplastic scar prevention and treatment .