CAJ | 학술논문

目的：探讨血清microRNA-17（miR-17）诊断早期宫颈鳞癌的价值及其与临床病理特征的关系。方法采集80例宫颈鳞癌患者及20例健康妇女的血清样本，采用实时RT-PCR测定血清miR-17水平，并绘制ROC曲线分析其诊断早期宫颈鳞癌的价值；分析血清miR-17水平与早期宫颈鳞癌临床病理因素的关系。结果早期宫颈鳞癌患者血清miR-17相对表达水平高于正常对照组（ P＜0．05）；ROC曲线下面积0．777，最大约登指数0．5，灵敏度0．95，特异度0．55，对应诊断界值0．858；血清miR-17相对表达水平与早期宫颈鳞癌的临床分期及病理分级有关（P＜0．05）；而与淋巴结转移、年龄、绝经状况无相关性（P＞0．05）。患者肿瘤分化程度越低、临床分期级别增加，血清miR-17水平越高。结论 miR-17在宫颈癌的发生、发展中发挥了作用，对早期宫颈鳞癌的筛查有一定价值。
목적：탐토혈청microRNA-17（miR-17）진단조기궁경린암적개치급기여림상병리특정적관계。방법채집80례궁경린암환자급20례건강부녀적혈청양본，채용실시RT-PCR측정혈청miR-17수평，병회제ROC곡선분석기진단조기궁경린암적개치；분석혈청miR-17수평여조기궁경린암림상병리인소적관계。결과조기궁경린암환자혈청miR-17상대표체수평고우정상대조조（ P＜0．05）；ROC곡선하면적0．777，최대약등지수0．5，령민도0．95，특이도0．55，대응진단계치0．858；혈청miR-17상대표체수평여조기궁경린암적림상분기급병리분급유관（P＜0．05）；이여림파결전이、년령、절경상황무상관성（P＞0．05）。환자종류분화정도월저、림상분기급별증가，혈청miR-17수평월고。결론 miR-17재궁경암적발생、발전중발휘료작용，대조기궁경린암적사사유일정개치。
Objective To evaluate the expression of serum microRNA-17(miR-17) in the diagnosis of early-stage squamous cervical cancer ,and to explore its correlation with clinicopathologic features of the disease .Methods The level of serum miR-17 was detected in 80 patients with squamous cervical cancer and 20 healthy women by real-time RT-PCR,and its diagnostic value was analyzed by receiver operating characteristic curve (ROC curve).The relationship between the level of serum miR-17 and the clinicopathologic features of the disease was analyzed .Results The relative expression level of serum miR-17 was significantly higher in patients with early-stage squamous cervical cancer in contrast with that in normal controls (P<0.01).The area under ROC curve was 0.777 and the max Youden index was 0.5,the sensitivity and specificity were 95%and 55%,respectively ,the cut-off point was 0.858.The relative expression level of miR-17 correlated with clinical stage and pathological grade of early -stage squamous cervical cancer (P<0.05), but didn′t correlate with lymph node metastasis ,the patients′age and menopausal status (P>0.05).Lower degree of tumor differentiation ,higher clinical stage and higher level of serum miR-17.Conclusion miR-17 plays a role in the occurrence and development of squamous cervical cancer ,and it is valuable for the screening of early-stage squamous cervical cancer .