CAJ | 학술논문

目的：探讨NIBP和NF-κB p65在胃癌中的表达及其临床意义。方法采用实时荧光定量PCR法、Western blot法和免疫组化SP法，检测55例胃癌、癌旁及正常胃组织中NIBP和NF-κB p65蛋白及mRNA的表达。结果胃癌组织中NIBP和NF-κB p65 mRNA和蛋白阳性表达率均高于癌旁及正常组织（ P＜0．05）；胃癌组织中NIBP和NF-κB p65的mRNA及蛋白相对表达水平均高于癌旁及正常组织（ P＜0．05）。胃癌组织中NIBP与NF-κB p65呈正相关（ P＜0．05）。胃癌组织中NIBP和NF-κB p65阳性表达率与肿瘤浸润程度、分化程度、有无远处转移及临床分期有关（ P＜0．05）。结论 NIBP和NF-κB p65在胃癌组织中高表达，可能参与了胃癌的发生和发展，且NIBP激活NF-κB p65信号通路途径可能在胃癌的浸润和转移过程中起重要作用。
목적：탐토NIBP화NF-κB p65재위암중적표체급기림상의의。방법채용실시형광정량PCR법、Western blot법화면역조화SP법，검측55례위암、암방급정상위조직중NIBP화NF-κB p65단백급mRNA적표체。결과위암조직중NIBP화NF-κB p65 mRNA화단백양성표체솔균고우암방급정상조직（ P＜0．05）；위암조직중NIBP화NF-κB p65적mRNA급단백상대표체수평균고우암방급정상조직（ P＜0．05）。위암조직중NIBP여NF-κB p65정정상관（ P＜0．05）。위암조직중NIBP화NF-κB p65양성표체솔여종류침윤정도、분화정도、유무원처전이급림상분기유관（ P＜0．05）。결론 NIBP화NF-κB p65재위암조직중고표체，가능삼여료위암적발생화발전，차NIBP격활NF-κB p65신호통로도경가능재위암적침윤화전이과정중기중요작용。
Objective To investigate the expression of NIK-and IKK2-binding protein ( NIBP) and nuclear factor-κB ( NF-κB ) p65 in gastric carcinoma and their clinical significance .Methods Real-time QT-PCR, immunohistochemistry and Western blot were used to detect the expressions of mRNA and protein of NIBP and NF-κB p65 in 55 cases of gastric carcinoma tissues ,adjacent tissues and normal tissues .Results The positive rates of mRNA and protein of NIBP and NF-κB were higher in gastric carcinoma tissues than those in adjacent tissues and normal tissues(P<0.05).The relative expression levels of mRNA and protein of NIBP and NF-κB p65 in gastric carcinoma tissues were significantly higher than those in adjacent tissues and normal tissues (P<0.05).There was a positive correlation between NIBP and NF-κB p65 in gastric carcinoma tissues .The positive expression of NIBP and NF-κB p65 in gastric carcinoma tissues was related with depth of invasion , differentiation , distant metastasis and clinical stage (P<0.05).Conclusion NIBP and NF-κB p65 are overexpression in gastric carcinoma tissues ,they might involve in the pathogenesis and progression of gastric carcinomar , and the NF-κB p65 signal pathway activated by NIBP might play an important role in the process of invasion and metastasis of gastric carcinoma .