CAJ | 학술논문

目的：探讨狼疮小鼠脾脏调节性T细胞（ Treg细胞）与狼疮样病征的相关性，为系统性红斑狼疮（SLE）的免疫疗法提供依据。方法选取22周龄雌性B6．MRL-Faslpr／J狼疮小鼠30只，留取24 h尿液及外周血后处死，取脾脏组织进行匀浆过滤，分别检测各小鼠24 h尿蛋白量、血清中抗核抗体（ ANA ）和抗ds-DNA抗体水平，流式细胞仪检测小鼠脾脏Treg细胞及淋巴细胞。应用Pearson对Treg细胞比例与24 h尿蛋白量、血清ANA和抗ds-DNA抗体水平进行相关分析。结果狼疮小鼠脾脏Treg 细胞比例与24 h 尿蛋白量及血清ds-DNA抗体水平均呈高度负相关关系（ P均＜0．01），与血清ANA水平呈负相关关系（ P＜0．05）。结论狼疮小鼠脾脏Treg细胞比例可能与狼疮样病征的活动性呈负相关关系。
목적：탐토랑창소서비장조절성T세포（ Treg세포）여랑창양병정적상관성，위계통성홍반랑창（SLE）적면역요법제공의거。방법선취22주령자성B6．MRL-Faslpr／J랑창소서30지，류취24 h뇨액급외주혈후처사，취비장조직진행균장과려，분별검측각소서24 h뇨단백량、혈청중항핵항체（ ANA ）화항ds-DNA항체수평，류식세포의검측소서비장Treg세포급림파세포。응용Pearson대Treg세포비례여24 h뇨단백량、혈청ANA화항ds-DNA항체수평진행상관분석。결과랑창소서비장Treg 세포비례여24 h 뇨단백량급혈청ds-DNA항체수평균정고도부상관관계（ P균＜0．01），여혈청ANA수평정부상관관계（ P＜0．05）。결론랑창소서비장Treg세포비례가능여랑창양병정적활동성정부상관관계。
Objective To investigate the correlation between splenic regulatory T cells (Treg cells) and lupus-like syndrome in lupus mice,and to provide the basis for the immune therapy of systemic lupus erythematosus (SLE).Methods Thirty 22-week-old female B6.MRL-Faslpr/J mice were selected ,they were killed after their 24-hour urine and peripheral blood had been collected ,the spleen tissue sample was made into homogenate and then was filtrated ,the levels of 24-hour urinary protein,serum antinuclear antibody(ANA) and anti-ds-DNA antibody of the mice were detected,flow cytometry was used to detect splenic Treg cells and lymphocytes of the mice .The correlation of the proportion of Treg cells with the levels of 24-hour urinary protein,serum ANA and anti-ds-DNA antibody was analyzed by Pearson correlation .Results The proportion of splenic Treg cells highly negatively correlated with the levels of 24-hour urinary protein and serum ds-DNA antibody in lupus mice(all P<0.01),and negatively correlated with the level of serum ANA (P<0.05).Conclusion The proportion of splenic Treg cells might negatively correlate with the activity of lupus -like syndrome in lupus mice .