动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
5期
117-123
,共7页
胡文举%宋艳画%秦佳辰%石奎林%于文浩%刘远哲%王新庄
鬍文舉%宋豔畫%秦佳辰%石奎林%于文浩%劉遠哲%王新莊
호문거%송염화%진가신%석규림%우문호%류원철%왕신장
奶牛%子宫内膜炎%致病菌%分离鉴定%药敏试验
奶牛%子宮內膜炎%緻病菌%分離鑒定%藥敏試驗
내우%자궁내막염%치병균%분리감정%약민시험
cow%endometritis%pathogenic bacteria%isolation and identification%drug sensitivity test
为调查河南平顶山地区奶牛场子宫内膜炎致病菌的种类、致病性和耐药性，采集了某牛场29头患子宫内膜炎奶牛的子宫内容物，进行细菌分离鉴定，小鼠攻毒试验及细菌药敏试验。共分离出蜡样芽胞杆菌、大肠埃希菌、奥斯陆莫拉菌等18种（52株）细菌，其中蜡样芽胞杆菌、奥斯陆莫拉菌、大肠埃希菌检出率较高，分别为41．4％（12/29）、20．7％（6/29）、17．2％（5/29）。小鼠致病性结果显示，大肠埃希菌对小鼠致病性最强，腹腔攻毒后2 d 内小鼠全部死亡；蜡样芽胞杆菌次之，腹腔攻毒后2 d 内致死率为75％，奥斯陆莫拉菌攻毒后2 d 内致死率为50％。药敏试验结果显示，蜡样芽胞杆菌、奥斯陆莫拉菌、大肠埃希菌均对阿莫西林和氨苄西林表现为耐药；对环丙沙星、恩诺沙星、强力霉素均表现为高度敏感。结果说明，蜡样芽胞杆菌、大肠埃希菌、奥斯陆莫拉菌为该地区奶牛子宫内膜炎主要致病菌；环丙沙星、恩诺沙星、强力霉素可作为该地区奶牛治疗子宫内膜炎的首选药物。
為調查河南平頂山地區奶牛場子宮內膜炎緻病菌的種類、緻病性和耐藥性，採集瞭某牛場29頭患子宮內膜炎奶牛的子宮內容物，進行細菌分離鑒定，小鼠攻毒試驗及細菌藥敏試驗。共分離齣蠟樣芽胞桿菌、大腸埃希菌、奧斯陸莫拉菌等18種（52株）細菌，其中蠟樣芽胞桿菌、奧斯陸莫拉菌、大腸埃希菌檢齣率較高，分彆為41．4％（12/29）、20．7％（6/29）、17．2％（5/29）。小鼠緻病性結果顯示，大腸埃希菌對小鼠緻病性最彊，腹腔攻毒後2 d 內小鼠全部死亡；蠟樣芽胞桿菌次之，腹腔攻毒後2 d 內緻死率為75％，奧斯陸莫拉菌攻毒後2 d 內緻死率為50％。藥敏試驗結果顯示，蠟樣芽胞桿菌、奧斯陸莫拉菌、大腸埃希菌均對阿莫西林和氨芐西林錶現為耐藥；對環丙沙星、恩諾沙星、彊力黴素均錶現為高度敏感。結果說明，蠟樣芽胞桿菌、大腸埃希菌、奧斯陸莫拉菌為該地區奶牛子宮內膜炎主要緻病菌；環丙沙星、恩諾沙星、彊力黴素可作為該地區奶牛治療子宮內膜炎的首選藥物。
위조사하남평정산지구내우장자궁내막염치병균적충류、치병성화내약성，채집료모우장29두환자궁내막염내우적자궁내용물，진행세균분리감정，소서공독시험급세균약민시험。공분리출사양아포간균、대장애희균、오사륙막랍균등18충（52주）세균，기중사양아포간균、오사륙막랍균、대장애희균검출솔교고，분별위41．4％（12/29）、20．7％（6/29）、17．2％（5/29）。소서치병성결과현시，대장애희균대소서치병성최강，복강공독후2 d 내소서전부사망；사양아포간균차지，복강공독후2 d 내치사솔위75％，오사륙막랍균공독후2 d 내치사솔위50％。약민시험결과현시，사양아포간균、오사륙막랍균、대장애희균균대아막서림화안변서림표현위내약；대배병사성、은낙사성、강력매소균표현위고도민감。결과설명，사양아포간균、대장애희균、오사륙막랍균위해지구내우자궁내막염주요치병균；배병사성、은낙사성、강력매소가작위해지구내우치료자궁내막염적수선약물。
Bacterial isolation and identification and drug sensitive test were conducted on intrauterine con-tents of 29 cows with endometritis in a dairy farm in Pingdingshan.There were 18 species(52 strins)bacte-ria in the 29 samples,such as Bacillus cereus ,E.coli,Moraxella osloensis ,in which Bacillus cereus , Moraxella osloensis and E.coli had the highest detection rates with 41.4%(12/29),20.7% (6/29)and 17.2% (5/29),respectively;Results of pathogenicity tests in mice showed that E.coli,Chryseobacterium meningosepticum,Streptococcus dysgalactiae ,Bacillus thuringiensis and Chryseobacterium indologenes had the strongest pathogenicity,with all of the mice died in 2 days;Bacillus cereus came second,with 3 mice died in 2 days after abdominal injection and the fatality rate was 75%,Moraxella osloensis came third in 2 days after,the fatality rate was 50%.Drug sensitivity tests showed that Bacillus cereus ,Moraxella osloensis ,E.coli showed drug resistance to amoxicillin and ampicillin but were highly sensitive to cipro-floxacin,enrofloxacin and doxycycline.The result showed that Bacillus cereus ,E.coli,Moraxella os-loensis were the main pathogenic bacteria of cow endometritis.Enrofloxacin,ciprofloxacin,doxycycline could be used as preferred drugs in treating endometritis in this farm.
