动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
5期
85-89
,共5页
苏双良%王慧%李洪萍%白秀娟
囌雙良%王慧%李洪萍%白秀娟
소쌍량%왕혜%리홍평%백수연
土鳖虫%肠道菌群%总DNA 提取%ERIC-PCR
土鱉蟲%腸道菌群%總DNA 提取%ERIC-PCR
토별충%장도균군%총DNA 제취%ERIC-PCR
Eupolyphaga sinesis%intestinal microflora%total DNA extraction%ERIC-PCR
比较不同方法提取土鳖虫肠道菌群总 DNA 的差异，并对土鳖虫肠道菌群组成多样性进行分析。采用试剂盒法和 CTAB 法提取土鳖虫肠道菌群总 DNA，检测其浓度和纯度，扩增16 S DNA 进行鉴定，并利用 ERIC-PCR 技术对土鳖虫肠道菌群多样性进行比较分析。结果显示，两种方法均能提取 DNA，用于16 S DNA 的扩增和 ERIC-PCR 分析，可用于进行土鳖虫肠道菌群的分子生态研究，CTAB 法更能反应土鳖虫肠道菌群的多样性。
比較不同方法提取土鱉蟲腸道菌群總 DNA 的差異，併對土鱉蟲腸道菌群組成多樣性進行分析。採用試劑盒法和 CTAB 法提取土鱉蟲腸道菌群總 DNA，檢測其濃度和純度，擴增16 S DNA 進行鑒定，併利用 ERIC-PCR 技術對土鱉蟲腸道菌群多樣性進行比較分析。結果顯示，兩種方法均能提取 DNA，用于16 S DNA 的擴增和 ERIC-PCR 分析，可用于進行土鱉蟲腸道菌群的分子生態研究，CTAB 法更能反應土鱉蟲腸道菌群的多樣性。
비교불동방법제취토별충장도균군총 DNA 적차이，병대토별충장도균군조성다양성진행분석。채용시제합법화 CTAB 법제취토별충장도균군총 DNA，검측기농도화순도，확증16 S DNA 진행감정，병이용 ERIC-PCR 기술대토별충장도균군다양성진행비교분석。결과현시，량충방법균능제취 DNA，용우16 S DNA 적확증화 ERIC-PCR 분석，가용우진행토별충장도균군적분자생태연구，CTAB 법경능반응토별충장도균군적다양성。
To screen a method that could provide a high quality DNA template and investigate the similarity and diversity of intestinal microflora in Eupolyphaga sinesis,TIANamp Stool DNA Kit method and CTAB method were used to extract total DNA from intestinal microflora of the Eupolyphaga sinesis.The DNA quality was compared based on the concentration,purity and 16 S DNA PCR products,and analysis of intestinal microflora was carried out by ERIC-PCR fingerprint.The results showed that total DNA ex-tracted by each of the two methods could be used to 16 S DNA amplification and ERIC-PCR fingerprint a-nalysis.The quality of total DNA extracted by the two methods was good enough to study molecular ecol-ogy of intestinal microflora.But the result of ERIC-PCR of DNA extracted by CTAB method showed more microflora diversities.
