动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2014年
5期
40-43
,共4页
康元环%孟庆峰%夏京津%龙继兵%盛宇轩%陈龙%单晓枫%钱爱东
康元環%孟慶峰%夏京津%龍繼兵%盛宇軒%陳龍%單曉楓%錢愛東
강원배%맹경봉%하경진%룡계병%성우헌%진룡%단효풍%전애동
乌鳢%维氏气单胞菌%分离鉴定%生物学特性
烏鱧%維氏氣單胞菌%分離鑒定%生物學特性
오례%유씨기단포균%분리감정%생물학특성
Channa argus%Aeromonas veronii%isolation and identification%biological characteristics
从患病乌鳢体内分离出一株细菌 WL161，人工感染试验显示，该菌株具有较强的毒力。经理化特性及16 S rRNA 基因序列分析，该病原菌为维氏气单胞菌（Aeromonas veronii ）。药敏试验结果显示，头孢克肟、头孢曲松钠等药物对菌株 WL161有较好的抑菌效果；部分毒力基因的检测结果证实，该菌携带气溶素、细胞毒性肠毒素、丝氨酸蛋白酶、黏附素、LuxS 等多种毒力基因。
從患病烏鱧體內分離齣一株細菌 WL161，人工感染試驗顯示，該菌株具有較彊的毒力。經理化特性及16 S rRNA 基因序列分析，該病原菌為維氏氣單胞菌（Aeromonas veronii ）。藥敏試驗結果顯示，頭孢剋肟、頭孢麯鬆鈉等藥物對菌株 WL161有較好的抑菌效果；部分毒力基因的檢測結果證實，該菌攜帶氣溶素、細胞毒性腸毒素、絲氨痠蛋白酶、黏附素、LuxS 等多種毒力基因。
종환병오례체내분리출일주세균 WL161，인공감염시험현시，해균주구유교강적독력。경이화특성급16 S rRNA 기인서렬분석，해병원균위유씨기단포균（Aeromonas veronii ）。약민시험결과현시，두포극우、두포곡송납등약물대균주 WL161유교호적억균효과；부분독력기인적검측결과증실，해균휴대기용소、세포독성장독소、사안산단백매、점부소、LuxS 등다충독력기인。
A bacterial strain named WL161 was isolated from the diseased Channa argus .The test on artifi-cial infected samples showed that WL161 had strong virulence.WL161 was identified as Aeromonas veronii by analysing 16 S rRNA gene sequence and physiological and biochemical tests.The drug sensitivity test indicated that WL161 was sensitive to eleven drugs.The virulence genes related to aerolysin(aer),cytolyt-ic enterotoxin (act),serine protease(ser),adhesin(Aha)and LuxS were detected in this research.
