CAJ | 학술논문

为鉴定小熊猫 IFN-γ原核表达产物的免疫活性，以纯化的小熊猫 IFN-γ原核表达产物为材料，采用淋巴细胞增殖试验和 ELISA，分别测定了 IFN-γ对小熊猫淋巴细胞增殖及其分泌 IFN-γ的影响。结果显示，与对照组相比，100μL 浓度分别为10-1×1．56 mg/mL、10-2×1．56 mg/mL 的纯化 IFN-γ能够显著刺激淋巴细胞增殖（P ＜0．05）；经热变性处理（煮沸10 min）、浓度为10-1×1．56 mg/mL 的 IFN-γ刺激淋巴细胞增殖的效果与空白对照无显著差异（P ＞0．05），但明显低于未处理组（P ＜0．05）；淋巴细胞培养系统中加入浓度为10-1×1．56 mg/mL 的等体积 IFN-γ，60 h 之内培养上清中的 IFN-γ含量均显著高于对照组（P＜0．01或 P ＜0．05）。结果表明，原核表达的小熊猫 IFN-γ产物具有刺激同种淋巴细胞增殖及分泌 IFN-γ的活性。
위감정소웅묘 IFN-γ원핵표체산물적면역활성，이순화적소웅묘 IFN-γ원핵표체산물위재료，채용림파세포증식시험화 ELISA，분별측정료 IFN-γ대소웅묘림파세포증식급기분비 IFN-γ적영향。결과현시，여대조조상비，100μL 농도분별위10-1×1．56 mg/mL、10-2×1．56 mg/mL 적순화 IFN-γ능구현저자격림파세포증식（P ＜0．05）；경열변성처리（자비10 min）、농도위10-1×1．56 mg/mL 적 IFN-γ자격림파세포증식적효과여공백대조무현저차이（P ＞0．05），단명현저우미처리조（P ＜0．05）；림파세포배양계통중가입농도위10-1×1．56 mg/mL 적등체적 IFN-γ，60 h 지내배양상청중적 IFN-γ함량균현저고우대조조（P＜0．01혹 P ＜0．05）。결과표명，원핵표체적소웅묘 IFN-γ산물구유자격동충림파세포증식급분비 IFN-γ적활성。
To identify the activities of prokaryotic expression IFN-γ in red panda,lymphocyte proliferation test and ELISA were used to investigate the role of IFN-γon PBMC proliferation and IFN-γ secretion re-spectively.The results showed that PBMC proliferation could be dramatically enhanced by adding 100 μL purified IFN-γwith concentrations of 10-1 ×1.56 mg/mL and 10 -2 ×1.56 mg/mL compared with control (P <0.05),while heat-denatured IFN-γwith a concentration of 10 -1 ×1.56 mg/mL had no effect on PB-MC proliferation and no significant difference with control (P >0.05).When culturing under purified IFN-γ stimulation at 10-1 ×1.56 mg/mL,the IFN-γ level in the supernatant was significantly higher than that of control within 60 h (P <0.01 or P <0.05).In conclusion,the purified IFN-γcould stimulate the prolif-eration and IFN-γ secretion of homologous PBMC.