CAJ | 학술논문

分别克隆凡纳滨对虾 Hsp70的 C 端结构域基因与对虾白斑综合征病毒（WSSV）VP28基因，构建融合基因并在大肠埃希菌中表达，用纯化融合蛋白免疫对虾，研究其对对虾的免疫保护作用和体内免疫相关基因表达的影响。结果表明，接种融合蛋白7 d 后，试验组对虾超氧化物歧化酶、溶菌酶、过氧化氢酶和 STAT 基因的表达量比对照组有显著提高，WSSV 感染试验表明，该融合蛋白延缓了对虾的死亡。
분별극륭범납빈대하 Hsp70적 C 단결구역기인여대하백반종합정병독（WSSV）VP28기인，구건융합기인병재대장애희균중표체，용순화융합단백면역대하，연구기대대하적면역보호작용화체내면역상관기인표체적영향。결과표명，접충융합단백7 d 후，시험조대하초양화물기화매、용균매、과양화경매화 STAT 기인적표체량비대조조유현저제고，WSSV 감염시험표명，해융합단백연완료대하적사망。
The C-terminal Hsp70 peptide-binding domain of Litopenaeus vannamei and white spot syndrome virus VP28 were cloned and ligated to the vector pBAD/gⅢA to constitute fusion gene.The fusion protein was expressed in E.coli Top10 and purified fusion protein was injected into the Litopenaeus vannamei . The protective effect and transcriptional expression changes of related gene were assayed.The results showed that the transcriptional expressions of SOD,LZM,CAT and STAT in the group injected with fu-sion protein are significantly enhanced compared with that of the control group.Administration of VP28 and Hsp70p fusion protein could delay the death of Litopenaeus vannamei from white spot disease.