CAJ | 학술논문

对传染性法氏囊病毒聊城分离株 VP2基因序列分析，为进一步开展 IBDV 诊断试剂及基因工程疫苗研究奠定基础。从山东聊城地区某养鸡场疑似鸡传染性法氏囊病病鸡初步分离到一株记为聊城分离株的 IBDV 毒株，从该分离株中提取总 RNA，采用 RT-PCR 技术扩增 VP2基因。测序结果与 GenBank 中报道的 IBDV VP2序列同源性在79．2％～98．7％。将 VP2基因序列与 GenBanK 上报道的22个序列进行系统发育进化树分析，证实 VP2基因与 SDH1株同源性最近。
대전염성법씨낭병독료성분리주 VP2기인서렬분석，위진일보개전 IBDV 진단시제급기인공정역묘연구전정기출。종산동료성지구모양계장의사계전염성법씨낭병병계초보분리도일주기위료성분리주적 IBDV 독주，종해분리주중제취총 RNA，채용 RT-PCR 기술확증 VP2기인。측서결과여 GenBank 중보도적 IBDV VP2서렬동원성재79．2％～98．7％。장 VP2기인서렬여 GenBanK 상보도적22개서렬진행계통발육진화수분석，증실 VP2기인여 SDH1주동원성최근。
The foundation was laid to carry out diagnostic reagents and genetically engineered vaccine re-search of IBDV.IBDV liaocheng isolate was isolated from suspected IBD chickens from a chicken farm in Liaocheng city.Viral dsRNA was extracted from the isolate,VP2 gene was acquired by RT-PCR.The ho-mology of the sequencing results was between 79.2% and 98.7% campared with sequences of IBDV VP2 reported from GenBank.Phylogenetic tree analysis between the VP2 gene and 22 sequenced VP2 gene re-ported from GenBank,confirmed the closest homology with SDH1 strain.