CAJ | 학술논문

目的：观察腺病毒（Ad-11β-HSD1）过表达系统及特异性siRNA（siRNA-11β-HSD1）干扰人肝癌HepG2细胞对11β-羟基类固醇脱氢酶I（11β-HSD1）表达的影响。方法应用Ad-11β-HSD1以及siRNA-11β-HSD1转染人肝癌HepG2细胞，用实时荧光定量PCR及Western Blot 检测11β-HSD1表达。结果转染72 h后，对照组及腺病毒过表达组细胞中均可见明显 GFP荧光，且转染48 h后蛋白水平明显升高；siRNA干扰48 h后，mRNA及蛋白水平均明显降低。结论腺病毒转染可有效过表达11β-HSD1，而siRNA干扰可有效抑制11β-HSD1表达。
목적：관찰선병독（Ad-11β-HSD1）과표체계통급특이성siRNA（siRNA-11β-HSD1）간우인간암HepG2세포대11β-간기류고순탈경매I（11β-HSD1）표체적영향。방법응용Ad-11β-HSD1이급siRNA-11β-HSD1전염인간암HepG2세포，용실시형광정량PCR급Western Blot 검측11β-HSD1표체。결과전염72 h후，대조조급선병독과표체조세포중균가견명현 GFP형광，차전염48 h후단백수평명현승고；siRNA간우48 h후，mRNA급단백수평균명현강저。결론선병독전염가유효과표체11β-HSD1，이siRNA간우가유효억제11β-HSD1표체。
Objective To design a adenovirus vector based system and specific siRNA for exploring the effects of overexpression and siRNA interference of the human hepatocellular carcinoma cell line HepG 2 on the ex-pression of 11β-hydroxysteroid dehydrogenase 1.Methods A adenovirus overexpression vector and specific siRNA targeting the 11β-HSD1 gene was designed and transfected into HepG 2 cells.Expression of 11β-HSD1 mRNA and protein was analyzed by real time PCR and Western blotting .Results A adenovirus transfection resulted in upgrade of 11β-HSD1 protein, and siRNA transfection led to inhibition of 11β-HSD1 mRNA and protein.Conclusion Trans-fection of the hepatocellular carcinoma cell line HepG 2 using a adenovirus vector and siRNA interference can signifi-cantly influence expression of 11β-HSD1 mRNA and protein .