CAJ | 학술논문

目的：观察转染睾丸特异性蛋白1（ TSPY1）慢病毒表达载体对肝癌细胞SMMC7721和Huh7细胞系增殖能力的影响。方法构建特异性的TSPY1慢病毒过表达载体稳定转染至SMMC7721和Huh7肝癌细胞系，采用荧光定量PCR和蛋白印迹技术检测TSPY1 mRNA和蛋白的表达情况，Cell Counting Kit-8（CCK-8）实验检测转染后细胞的增殖情况。结果在转染过表达TSPY1慢病毒的SMMC7721和 Huh7细胞中， TSPY1 mRNA和蛋白的表达均明显上调（ P＜0.01）。 CCK-8细胞增殖实验发现，过表达TSPY1组SMMC7721和Huh7细胞的增殖能力比对照组增强。结论 TSPY1慢病毒表达载体可明显上调TSPY1 mRNA和蛋白表达，并能够促进肝癌SMMC7721和Huh7细胞的增殖。
목적：관찰전염고환특이성단백1（ TSPY1）만병독표체재체대간암세포SMMC7721화Huh7세포계증식능력적영향。방법구건특이성적TSPY1만병독과표체재체은정전염지SMMC7721화Huh7간암세포계，채용형광정량PCR화단백인적기술검측TSPY1 mRNA화단백적표체정황，Cell Counting Kit-8（CCK-8）실험검측전염후세포적증식정황。결과재전염과표체TSPY1만병독적SMMC7721화 Huh7세포중， TSPY1 mRNA화단백적표체균명현상조（ P＜0.01）。 CCK-8세포증식실험발현，과표체TSPY1조SMMC7721화Huh7세포적증식능력비대조조증강。결론 TSPY1만병독표체재체가명현상조TSPY1 mRNA화단백표체，병능구촉진간암SMMC7721화Huh7세포적증식。
Objective To investigate the effects of lentivirus vector-Testis-specific Protein Y-encoded 1 (TSPY1)on the proliferation ability of SMMC7721 and Huh7 hepatocellular carcinoma cells .Methods The lentivir-us vector was constructed and transfected into the SMMC 7721 and Huh7 cells.The expression of TSPY1 mRNA and protein was detected by quantitative RT-PCR and western blot .Cell Counting Kit-8 ( CCK8 ) assay was performed to assess the transfected cells proliferation .Results The expression of TSPY1 both in mRNA and protein levels were significantly increased in the SMMC 7721 and Huh7 cells transfected with lentivirus encoding TSPY 1 cDNA ( P <0.01 ) .CCK8 assay indicated that TSPY 1 potentiates cell proliferation both in SMMC 7721 and Huh7 cells over-ex-pressed TSPY1, comparing to the control cells .Conclusion The transfection of lentiviralvector-mediated TSPY1 gene can obviously up-regulate the expression of TSPY 1 mRNA and protein and promote the proliferation of SMMC7721 and Huh hepatocellular carcinoma cells .