CAJ | 학술논문

目的探讨肾素前体（prorenin）诱导大鼠血管平滑肌细胞的增殖是否通过ERK1/2和Akt通路。方法利用[3H]同位素标记的胸苷的结合检测血管平滑肌细胞增殖。蛋白印记分析检测肾素前体诱导的平滑肌细胞ERK和Akt的活化。结果肾素前体剂量依赖性诱导ERK和Akt磷酸化，在20min达到高峰。20nmol/L肾素前体处理细胞48h后细胞内[3H]同位素标记的胸苷含量增多（P＜0.05），这种促进细胞增殖的作用可以被预处理ERK激酶抑制剂PD98059（100μmol/L）和PI3K激酶抑制剂wortmannin（100nmol/L）阻断。结论实验结果表明肾素前体诱导血管平滑肌细胞产生的增殖是通过ERK1/2和Akt通路发挥作用。
목적탐토신소전체（prorenin）유도대서혈관평활기세포적증식시부통과ERK1/2화Akt통로。방법이용[3H]동위소표기적흉감적결합검측혈관평활기세포증식。단백인기분석검측신소전체유도적평활기세포ERK화Akt적활화。결과신소전체제량의뢰성유도ERK화Akt린산화，재20min체도고봉。20nmol/L신소전체처리세포48h후세포내[3H]동위소표기적흉감함량증다（P＜0.05），저충촉진세포증식적작용가이피예처리ERK격매억제제PD98059（100μmol/L）화PI3K격매억제제wortmannin（100nmol/L）조단。결론실험결과표명신소전체유도혈관평활기세포산생적증식시통과ERK1/2화Akt통로발휘작용。
Objective To investigate the effect of prorenin on vascular smooth muscle cell(VSMC) proliferation via ERK and Akt activation dependent pathway.Methods DNA synthesis in rat VSMCs was measuring by [3H] thymidine.Extracellular signal-regulated kinase(ERK) and Akt phosphorylation were measured by Western blotting.Results Rat recombinant prorenin dose-dependently induced ERK and Akt phosphorylation with a peak at 20min. Prorenin(20nmol/L,48h) significantly increase VSMC [3H] thymidine incorporation,which attenuated by pretreatment with ERK kinase inhibitor,PD98059(100μmol/L),and PI3 kinase inhibitors,wortmannin(100nmol/L).Conclusion ERK and Akt activation contributes to prorenin-induced proliferative effects in VSMCs.