CAJ | 학술논문

利用反相悬浮结合溶剂结晶致孔法制备了具有超大孔隙、球形度良好的聚丙烯酰胺晶胶微球，晶胶微球平均粒径为234.1μm，孔径约为10～50μm。采用原位接枝法，将2-丙烯酰胺基-2-甲基-1-丙烷磺酸（AMPSA）接枝到晶胶微球孔隙表面上，得到了带有磺酸基团的阳离子交换晶胶微球。考察了晶胶微球的生物相容性和吸附蛋白质及重金属离子Cu2+的性能，结果表明：在大肠杆菌培养液中，添加晶胶微球，对大肠杆菌的生长影响不大。接枝了AMPSA的晶胶微球表现出更强的吸附Cu2+的能力，吸附容量达到1.14 mmol·g-1。同时，接枝后的晶胶微球也具有一定吸附蛋白质的能力，溶菌酶的吸附容量达到54.5 mg·g-1。因此，该大孔晶胶可望在微生物固定化、生物分离和重金属离子吸附中会发挥更大的作用。
이용반상현부결합용제결정치공법제비료구유초대공극、구형도량호적취병희선알정효미구，정효미구평균립경위234.1μm，공경약위10～50μm。채용원위접지법，장2-병희선알기-2-갑기-1-병완광산（AMPSA）접지도정효미구공극표면상，득도료대유광산기단적양리자교환정효미구。고찰료정효미구적생물상용성화흡부단백질급중금속리자Cu2+적성능，결과표명：재대장간균배양액중，첨가정효미구，대대장간균적생장영향불대。접지료AMPSA적정효미구표현출경강적흡부Cu2+적능력，흡부용량체도1.14 mmol·g-1。동시，접지후적정효미구야구유일정흡부단백질적능력，용균매적흡부용량체도54.5 mg·g-1。인차，해대공정효가망재미생물고정화、생물분리화중금속리자흡부중회발휘경대적작용。
Novel supermacropore cryogel beads were prepared by combining the inverse-suspension and cryo-polymerization methods under frozen condition. The mean diameter of the beads was 234.1μm, and the pore size was 10-50 μm. The cation-exchange cryogel beads were prepared by in-situ grafting of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) onto the pore surface of the cryogel beads. The biocompatibility of the cryogel beads was measured. The adsorption of protein and Cu2+on the cryogel beads was also discussed. The growth of E.coli was little affected when cryogel beads existed in the cultivation. The cryogel beads had stronger adsorption ability after grafting AMPSA, and the adsorption capacity of Cu2+ could reach 1.14 mmol·g-1. Meanwhile, the cryogel beads grafted with AMPSA had the ability of protein adsorption, and the adsorption capacity for lysozyme reached 54.5 mg · g-1. Such cryogel beads could be used in immobilization of microorganisms, separation of biomass components and adsorption of heavy metal ions.