CAJ | 학술논문

　　建立高效液相色谱测定剑麻麻膏、麻膏水解物和剑麻皂素产品中剑麻皂甙元含量的分析方法。色谱条件采用示差折光检测器，C18柱和40℃的柱温，以乙醇-水（3+1，v/v）为流动相。实验结果显示，剑麻皂甙元在0.002~0.04mg范围内呈现良好的线性关系，其线性相关系数为0.9998，该方法对3种样品的平均回收率分别为97.1%、97.8%、98.9%，RSD（n=5）分别为0.81%、0.65%、0.51%。该方法简便、准确、重现性好，可为剑麻皂素生产中的原料采购和产品质量评价提供有效依据。
　　건립고효액상색보측정검마마고、마고수해물화검마조소산품중검마조대원함량적분석방법。색보조건채용시차절광검측기，C18주화40℃적주온，이을순-수（3+1，v/v）위류동상。실험결과현시，검마조대원재0.002~0.04mg범위내정현량호적선성관계，기선성상관계수위0.9998，해방법대3충양품적평균회수솔분별위97.1%、97.8%、98.9%，RSD（n=5）분별위0.81%、0.65%、0.51%。해방법간편、준학、중현성호，가위검마조소생산중적원료채구화산품질량평개제공유효의거。
A method for the determination of tigogenin in fermented residues of sisal leaf-juices, sisal hydrolysate and sisal saponin product by HPLC with RID was established. C18 column was used as stationary phase and ethanol-water(3+1, v/v) as mobile phase, the flow rate was 1.0mL/min and the column temperature was 40℃. The calibration curve was linear in the range of 0.002~0.04mg (R2=0.9998). The average recovery were 97.1%(RSD=0.81%), 97.8%( RSD=0.65%) and 98.9%( RSD=0.51%) for the three kind of samples. The method was convenient, stable and accurate, so it could provide the effective proof for raw material purchase and quality evaluation in sisal saponin production.