防护林科技
防護林科技
방호림과기
PROTECTION FOREST SCIENCE AND TECHNOLOGY
2014年
6期
9-10,28
,共3页
山杨%美洲黑杨%正己烷%花粉提取液%杂交障碍
山楊%美洲黑楊%正己烷%花粉提取液%雜交障礙
산양%미주흑양%정기완%화분제취액%잡교장애
Populus davidiana%Populus deltoides%n-hexane%the fluid extracted from pollen%hybridization barriers
以山杨为母本、美洲黑杨为父本，对克服山杨与美洲黑杨派间杂交障碍技术进行研究。结果表明：用正己烷有机溶剂熏柱头后再用山杨花粉提取液处理柱头能有效地克服山杨与美洲黑杨之间的杂交障碍。同时，用形态学标记对杂交子代及其亲本进行鉴定分析，杂种子代主要偏向于父本型与母本型，从而证明杂种子代是其真正的杂种。
以山楊為母本、美洲黑楊為父本，對剋服山楊與美洲黑楊派間雜交障礙技術進行研究。結果錶明：用正己烷有機溶劑熏柱頭後再用山楊花粉提取液處理柱頭能有效地剋服山楊與美洲黑楊之間的雜交障礙。同時，用形態學標記對雜交子代及其親本進行鑒定分析，雜種子代主要偏嚮于父本型與母本型，從而證明雜種子代是其真正的雜種。
이산양위모본、미주흑양위부본，대극복산양여미주흑양파간잡교장애기술진행연구。결과표명：용정기완유궤용제훈주두후재용산양화분제취액처리주두능유효지극복산양여미주흑양지간적잡교장애。동시，용형태학표기대잡교자대급기친본진행감정분석，잡충자대주요편향우부본형여모본형，종이증명잡충자대시기진정적잡충。
Taking Populus davidiana as female parent and Populus deltoides as male parent ,technology for overco-ming the crossing barrier between P .davidiana& P .deltoides were studied .Result shows that suffocating stigma with n-hexane and bushing stigma with the fluid extracted from pollen of P .davidiana can effectively overcome in-compatible barriers between P .davidiana& P .deltoides .At the same time ,the hybrids & their parents were i-dentified by using morphological marker ;hybrids offspring are inclined to male parent & female parent ;thus proves that its hybrid offspring are its true hybrids .
