中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2014年
7期
9-13,20
,共6页
Snail%E-cadherin%Vimentin%上皮性卵巢癌%上皮间质转化
Snail%E-cadherin%Vimentin%上皮性卵巢癌%上皮間質轉化
Snail%E-cadherin%Vimentin%상피성란소암%상피간질전화
Snail%E-cadherin%Vimentin%Epithelial ovarian cancer%Epithelial-mesenchymal transition
目的：检测Snail、E-cadherin及Vimentin在上皮性卵巢癌组织中的表达，探讨Snail介导的上皮间质转化（EMT）在卵巢癌发生、发展及转移中的作用。方法采用免疫组化法分别检测Snail、E-cadherin、Vimentin在48例卵巢浆液性腺癌、卵巢交界性浆液性肿瘤及卵巢浆液性腺瘤中的表达，探讨EMT相关因子表达强度的相关性及与临床病理特征之间的关系。结果（1）Snail、Vimentin在卵巢浆液性腺癌中的表达率为（68.75%/66.67%），高于卵巢交界性浆液性腺瘤的(41.67%/37.50%)及卵巢浆液性腺瘤的(25.00%/18.75%)，结果均具有显著的统计学差异（P＜0.05），E-cadherin在卵巢浆液性腺癌中的表达率为27.08%，低于与卵巢交界性浆液性腺瘤的54.17%及卵巢浆液性腺瘤的75.00%，结果均具有统计学差异（P＜0.05）。而卵巢交界性浆液性腺瘤组与卵巢浆液性腺瘤组比较，三种蛋白的表达均无统计学差异（P＞0.05）。（2）Snail、E-cadherin及Vimentin的表达高低与FIGO分期、分化级别、有无淋巴结转移及腹膜种植有关。（3）在卵巢浆液性腺癌中，Snail与Vimentin的表达呈正相关（r=0.477，P＜0.05），Snail与E-cadherin的表达呈负相关（r=-0.601，P＜0.05），而E-cadherin与Vimentin表达的相关性不明显（r=-0.206, P＞0.05）。结论在上皮性卵巢癌组织中，Snail、Vimentin表达上调，E-cadherin表达下调，提示Snail介导的EMT可能与卵巢癌的发生、发展及浸润转移有关。
目的：檢測Snail、E-cadherin及Vimentin在上皮性卵巢癌組織中的錶達，探討Snail介導的上皮間質轉化（EMT）在卵巢癌髮生、髮展及轉移中的作用。方法採用免疫組化法分彆檢測Snail、E-cadherin、Vimentin在48例卵巢漿液性腺癌、卵巢交界性漿液性腫瘤及卵巢漿液性腺瘤中的錶達，探討EMT相關因子錶達彊度的相關性及與臨床病理特徵之間的關繫。結果（1）Snail、Vimentin在卵巢漿液性腺癌中的錶達率為（68.75%/66.67%），高于卵巢交界性漿液性腺瘤的(41.67%/37.50%)及卵巢漿液性腺瘤的(25.00%/18.75%)，結果均具有顯著的統計學差異（P＜0.05），E-cadherin在卵巢漿液性腺癌中的錶達率為27.08%，低于與卵巢交界性漿液性腺瘤的54.17%及卵巢漿液性腺瘤的75.00%，結果均具有統計學差異（P＜0.05）。而卵巢交界性漿液性腺瘤組與卵巢漿液性腺瘤組比較，三種蛋白的錶達均無統計學差異（P＞0.05）。（2）Snail、E-cadherin及Vimentin的錶達高低與FIGO分期、分化級彆、有無淋巴結轉移及腹膜種植有關。（3）在卵巢漿液性腺癌中，Snail與Vimentin的錶達呈正相關（r=0.477，P＜0.05），Snail與E-cadherin的錶達呈負相關（r=-0.601，P＜0.05），而E-cadherin與Vimentin錶達的相關性不明顯（r=-0.206, P＞0.05）。結論在上皮性卵巢癌組織中，Snail、Vimentin錶達上調，E-cadherin錶達下調，提示Snail介導的EMT可能與卵巢癌的髮生、髮展及浸潤轉移有關。
목적：검측Snail、E-cadherin급Vimentin재상피성란소암조직중적표체，탐토Snail개도적상피간질전화（EMT）재란소암발생、발전급전이중적작용。방법채용면역조화법분별검측Snail、E-cadherin、Vimentin재48례란소장액성선암、란소교계성장액성종류급란소장액성선류중적표체，탐토EMT상관인자표체강도적상관성급여림상병리특정지간적관계。결과（1）Snail、Vimentin재란소장액성선암중적표체솔위（68.75%/66.67%），고우란소교계성장액성선류적(41.67%/37.50%)급란소장액성선류적(25.00%/18.75%)，결과균구유현저적통계학차이（P＜0.05），E-cadherin재란소장액성선암중적표체솔위27.08%，저우여란소교계성장액성선류적54.17%급란소장액성선류적75.00%，결과균구유통계학차이（P＜0.05）。이란소교계성장액성선류조여란소장액성선류조비교，삼충단백적표체균무통계학차이（P＞0.05）。（2）Snail、E-cadherin급Vimentin적표체고저여FIGO분기、분화급별、유무림파결전이급복막충식유관。（3）재란소장액성선암중，Snail여Vimentin적표체정정상관（r=0.477，P＜0.05），Snail여E-cadherin적표체정부상관（r=-0.601，P＜0.05），이E-cadherin여Vimentin표체적상관성불명현（r=-0.206, P＞0.05）。결론재상피성란소암조직중，Snail、Vimentin표체상조，E-cadherin표체하조，제시Snail개도적EMT가능여란소암적발생、발전급침윤전이유관。
Objective To Study the effect on Snail mediated epithelial mesenchymal transition (EMT) in ovarian cancer occurrence, development and metastasis. Methods Immunohistochemical staining was detected Snail, E-cadherin,in 48 cases of ovarian serous adenocarcinoma,ovarian serous borderline tumors and ovarian serous adenomas Vimentin,investigate the correlation between the expression of EMT -related factors and the strength and relationship between clinicopathological features. Results(1)The expression of Snail,Vimentin in ovarian serous adenocarcinoma was 68.75% and 66.67%,higher than in borderline serous ovarian tumors (41.67% and 37.50) and in ovarian serous adenomas( 25.00%and 18.75%),with significant difference(P<0.05),the expression of E-cadherin in ovarian serous adenocarcinoma of the rate of 27.08%,lower than in borderline serous ovarian tumors(54.17%) and in ovarian serous adenomas(75.00%),with significant difference(P < 0.05). Between the borderline ovarian serous cystadenoma and ovarian serous adenoma group,there was statistical difference in the expression of the three proteins(P > 0.05).(2)The expression level of Snail and FIGO, E-cadherin and Vimentin staging,differentiation level,there was no lymph node metastasis and peritoneal implants.(3)in ovarian serous adenocarcinoma, the expression of Snail positively correlated with Vimentin (r=0.477,P < 0.05),the expression of Snail was negatively correlated with E-cadherin(r=-0.601,P < 0.05),and the expression of E-cadherin and Vimentin correlation is not obvious(r=-0.206,P< 0.05). Conclusion In epithelial ovarian cancer,Snail,Vimentin upregulation,E-cadherin downregulation,suggesting that Snail-mediated EMT may be transferred with ovarian cancer,development and infiltration.
