北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
6期
67-68,69
,共3页
林晓晖%黄玲%王一铮%刘如玉
林曉暉%黃玲%王一錚%劉如玉
림효휘%황령%왕일쟁%류여옥
酒精性肝损伤%动物模型
酒精性肝損傷%動物模型
주정성간손상%동물모형
Alcohol liver disease%Animal model
目的:建立小鼠酒精性肝损伤模型,并动态研究小鼠血清和肝脏的生化指标的变化。方法:将80只清洁级ICR小鼠随机分为8个组:4个模型组和4个空白组。模型组小鼠按12ml·kg-1d-1胃灌注52度白酒,空白组小鼠给予胃灌注等量蒸馏水,分别在灌胃后2d,4d,6d,8d各取一组模型组和空白组小鼠,测定血清ALT、TG,肝脏指数,肝组织SOD和MDA。结果:与同时段空白组比较,造模6d后,小鼠血清中ALT和TG均显著升高(P<0.01),肝脏指数、肝组织中TG和MDA显著升高(P<0.01),而SOD活性显著下降(P<0.01)。结论:本实验在短时间内成功建立小鼠急性酒精性肝损伤模型,稳定性好,可应用于护肝药物保护机制的研究。
目的:建立小鼠酒精性肝損傷模型,併動態研究小鼠血清和肝髒的生化指標的變化。方法:將80隻清潔級ICR小鼠隨機分為8箇組:4箇模型組和4箇空白組。模型組小鼠按12ml·kg-1d-1胃灌註52度白酒,空白組小鼠給予胃灌註等量蒸餾水,分彆在灌胃後2d,4d,6d,8d各取一組模型組和空白組小鼠,測定血清ALT、TG,肝髒指數,肝組織SOD和MDA。結果:與同時段空白組比較,造模6d後,小鼠血清中ALT和TG均顯著升高(P<0.01),肝髒指數、肝組織中TG和MDA顯著升高(P<0.01),而SOD活性顯著下降(P<0.01)。結論:本實驗在短時間內成功建立小鼠急性酒精性肝損傷模型,穩定性好,可應用于護肝藥物保護機製的研究。
목적:건립소서주정성간손상모형,병동태연구소서혈청화간장적생화지표적변화。방법:장80지청길급ICR소서수궤분위8개조:4개모형조화4개공백조。모형조소서안12ml·kg-1d-1위관주52도백주,공백조소서급여위관주등량증류수,분별재관위후2d,4d,6d,8d각취일조모형조화공백조소서,측정혈청ALT、TG,간장지수,간조직SOD화MDA。결과:여동시단공백조비교,조모6d후,소서혈청중ALT화TG균현저승고(P<0.01),간장지수、간조직중TG화MDA현저승고(P<0.01),이SOD활성현저하강(P<0.01)。결론:본실험재단시간내성공건립소서급성주정성간손상모형,은정성호,가응용우호간약물보호궤제적연구。
Objective:To establish an alcohol-induced acute liver injury model in mice and to dynamically monitor the changes of biochemical indexes in serum and liver. Methods :Eighty ICR mice were randomly divided into eight groups, four control groups and four model groups. The model groups were given 52%(V/V)alcohol with 12ml·kg-1d-1 by gastric perfusion while the control groups were given distilled water. At 2, 4, 6, 8 days after administration, a model group and a control group were respectively sacrificed to take serum samples for measurements of ALT and TG levels, and liver samples for measurements of liver index, TG, SOD and MDA levels. Results:After treating for 6 days, serum ALT and TG levels were significantly increased(P<0.01), and liver index, TG and MDA level in liver tissue were increased significantly(P<0.01), whereas SOD level in liver tissue decreased significantly(P<0.01). Conclusion :A mice model of alcohol-induced acute liver injury has been successfully established, which is stable and can be used for studying the mechanism of drug protection in alcoholic liver disease.