CAJ | 학술논문

　　目的采用双波长高效液相色谱法同时测定安坤种子丸中阿魏酸和芍药苷2种有效成分的含量。方法采用Waters SymmetryShield-C18柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速1.0 mL/min,检测波长分别为230、323 nm。结果阿魏酸、芍药苷线性范围分别为0.0582～0.5824μg(r＝0.9994)、1.664～16.64μg(r＝0.9996),平均加样回收率分别为97.77%(RSD＝1.88%)、98.84%(RSD＝1.96%)。结论所建立的方法可对安坤种子丸中阿魏酸和芍药苷准确、快速地进行定量检测,可用于其质量控制。
　　목적채용쌍파장고효액상색보법동시측정안곤충자환중아위산화작약감2충유효성분적함량。방법채용Waters SymmetryShield-C18주(4.6 mm×250 mm,5μm),이을정-0.1%린산용액위류동상제도세탈,류속1.0 mL/min,검측파장분별위230、323 nm。결과아위산、작약감선성범위분별위0.0582～0.5824μg(r＝0.9994)、1.664～16.64μg(r＝0.9996),평균가양회수솔분별위97.77%(RSD＝1.88%)、98.84%(RSD＝1.96%)。결론소건립적방법가대안곤충자환중아위산화작약감준학、쾌속지진행정량검측,가용우기질량공제。
Objective To determine ferulic acid and paeoniflorin in Ankong Zhongzi Wan by HPLC under dual wavelength ultraviolet detection. Methods Ferulic acid and paeoniflorin were separated by Waters SymmetryShield-C18 column (4.6 mm × 250 mm, 5 μm) with gradient elution of acetonitrile-0.1%phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 230 nm and 323 nm. Results The linear relationship of ferulic acid and paeoniflorin was good in the range of 0.058 2-0.582 4 μg (r=0.999 4) and 1.664-16.64 μg (r=0.999 6), and the average recovery rate was 97.77% (RSD=1.88%) and 98.84% (RSD=1.96%), respectively. Conclusion The method is accurate and quick for determining the two effective components in Ankong Zhongzi Wan, and can be used for its quality control.