中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
9期
47-48
,共2页
草乌%乌头碱%煎煮时间%高效液相色谱法
草烏%烏頭堿%煎煮時間%高效液相色譜法
초오%오두감%전자시간%고효액상색보법
Radix Aconiti Kusnezoffii%aconitine%decocting time%HPLC
目的考察生草乌在不同煎煮时间内毒性成分乌头碱的含量变化。方法采用反相高效液相色谱法测定乌头碱的含量,以甲醇-水-氯仿-二乙胺(70∶30∶3∶0.2)为流动相,流速1.0 mL/min,柱温30℃,紫外检测波长为240 nm。结果乌头碱在0.08～0.8μg范围内呈良好的线性关系,r＝0.9998(n＝6),平均回收率为97.16%。生草乌在煎煮过程中乌头碱的含量0～2 h内逐渐升高,2 h以后逐渐降低,2～4 h降低幅度尤为明显。结论高效液相色谱法测定乌头碱的含量快速、简便、准确、重复性好、结果可靠。生草乌煎煮4h能有效减低乌头碱的含量。
目的攷察生草烏在不同煎煮時間內毒性成分烏頭堿的含量變化。方法採用反相高效液相色譜法測定烏頭堿的含量,以甲醇-水-氯倣-二乙胺(70∶30∶3∶0.2)為流動相,流速1.0 mL/min,柱溫30℃,紫外檢測波長為240 nm。結果烏頭堿在0.08～0.8μg範圍內呈良好的線性關繫,r＝0.9998(n＝6),平均迴收率為97.16%。生草烏在煎煮過程中烏頭堿的含量0～2 h內逐漸升高,2 h以後逐漸降低,2～4 h降低幅度尤為明顯。結論高效液相色譜法測定烏頭堿的含量快速、簡便、準確、重複性好、結果可靠。生草烏煎煮4h能有效減低烏頭堿的含量。
목적고찰생초오재불동전자시간내독성성분오두감적함량변화。방법채용반상고효액상색보법측정오두감적함량,이갑순-수-록방-이을알(70∶30∶3∶0.2)위류동상,류속1.0 mL/min,주온30℃,자외검측파장위240 nm。결과오두감재0.08～0.8μg범위내정량호적선성관계,r＝0.9998(n＝6),평균회수솔위97.16%。생초오재전자과정중오두감적함량0～2 h내축점승고,2 h이후축점강저,2～4 h강저폭도우위명현。결론고효액상색보법측정오두감적함량쾌속、간편、준학、중복성호、결과가고。생초오전자4h능유효감저오두감적함량。
Objective To investigate the content change of aconitine in Radix Aconiti Kusnezoffii in different boiling time. Methods The chromatographic procedure was carried out with methanol-water-chloroform-diethylamide (70∶30∶3∶0.2) as mobile phase at a flow rate of 1.0 mL/min, the column temperature at 30 ℃, and UV detector at 240 nm. Results There was a good linear relationship of aconitine within 0.08-0.8 μg, r=0.999 8 (n=6), and the average recovery rate was 97.16%. The content of aconitine increased gradually in 0-2 h, then gradually decreased. And the reduction was evident particularly during 2-4 hours. Conclusion HPLC method is rapid, simple, accurate, reproducible and reliable for determining aconitine. The content of aconitine can be decreased effectively by boiling 4 hours.
