CAJ | 학술논문

　　目的观察电针“三阴穴”对慢性非细菌性前列腺炎大鼠模型免疫功能的影响,并探讨其作用机制。方法将48只雄性SD大鼠随机分为空白组、模型组、药物组、电针组。皮内注射同种异体雄性SD大鼠前列腺蛋白提纯液,辅以双重免疫佐剂复制自身免疫性慢性非细菌性前列腺炎大鼠模型。造模后电针组、药物组分别给予电针“三阴穴”、舍尼通灌胃治疗15 d。观察各组动物前列腺组织湿重及指数、形态结构,用放免法检测前列腺组织中抗体IgG含量,局部组织细胞因子白细胞介素(IL)-2、IL-8的变化。结果造模后模型组前列腺组织湿重及指数与空白组比较显著增高(P＜0.05),病理观察显示,模型组动物前列腺组织形态及结构明显受损；与空白组比较,模型组大鼠前列腺组织匀浆中IgG含量明显增高(P＜0.05),局部细胞因子IL-2、IL-8高表达(P＜0.05,P＜0.01)。治疗后,电针组前列腺湿重及指数均明显下降(P＜0.05,P＜0.01),病理变化明显减轻；局部前列腺组织IgG含量明显降低(P＜0.05),局部细胞因子IL-2、IL-8表达显著下降(P＜0.05,P＜0.01)。结论电针“三阴穴”能够减少非细菌性前列腺炎大鼠局部抗体IgG的分泌,调节局部细胞因子IL-2、IL-8的表达,减轻组织局部免疫亢进,抑制前列腺组织形态结构的损伤,从而起到治疗慢性非细菌性前列腺炎的作用。
　　목적관찰전침“삼음혈”대만성비세균성전렬선염대서모형면역공능적영향,병탐토기작용궤제。방법장48지웅성SD대서수궤분위공백조、모형조、약물조、전침조。피내주사동충이체웅성SD대서전렬선단백제순액,보이쌍중면역좌제복제자신면역성만성비세균성전렬선염대서모형。조모후전침조、약물조분별급여전침“삼음혈”、사니통관위치료15 d。관찰각조동물전렬선조직습중급지수、형태결구,용방면법검측전렬선조직중항체IgG함량,국부조직세포인자백세포개소(IL)-2、IL-8적변화。결과조모후모형조전렬선조직습중급지수여공백조비교현저증고(P＜0.05),병리관찰현시,모형조동물전렬선조직형태급결구명현수손；여공백조비교,모형조대서전렬선조직균장중IgG함량명현증고(P＜0.05),국부세포인자IL-2、IL-8고표체(P＜0.05,P＜0.01)。치료후,전침조전렬선습중급지수균명현하강(P＜0.05,P＜0.01),병리변화명현감경；국부전렬선조직IgG함량명현강저(P＜0.05),국부세포인자IL-2、IL-8표체현저하강(P＜0.05,P＜0.01)。결론전침“삼음혈”능구감소비세균성전렬선염대서국부항체IgG적분비,조절국부세포인자IL-2、IL-8적표체,감경조직국부면역항진,억제전렬선조직형태결구적손상,종이기도치료만성비세균성전렬선염적작용。
Objective To study the immunologic mechanism of electroacupuncture at Sanyin points on chronic abacterial prostatitis rats. Methods Forty-eight male rats were divided randomly into normal group, model group, drug group and electroacupuncture group, 12 rats in each group. The animal model was made by SC purified prostate protein twice with immunologic adjuvant, and the rats in electroacupuncture group and drug group were treated by electroacupuncture at Sanyin points and feeding Cernilton for 15 days. The changes of prostate weight, index, pathology and contents of IgG, expressions of IL-2 and IL-8 in prostate homogenate were observed. Results Compared with normal group, the prostate weight and index were significantly increased in model group (P<0.05), the content of IgG was significantly increased (P<0.05), cytokine IL-2 and IL-8 were highly expressed (P<0.05, P<0.01), prostate tissue’s morphology and structure were significantly impaired in model group. Compared with model group, the prostate weight and index were significantly decreased in electroacupuncture group after treatment (P<0.05 or P<0.01), pathological changes reduced significantly, the content of IgG decreased significantly (P<0.05), the expressions of cytokine IL-2 and IL-8 were significantly decreased (P<0.05, P<0.01), and the pathological manifestation of chronic inflammation was significantly improved. Conclusion Electroacupuncture at Sanyin points can reduce the secretion of local prostate antibody IgG, regulate the expression of cytokine IL-2 and IL-8, protect the morphology and structure of prostate tissue from damage. Electroacupuncture at Sanyin points can adjust local immunologic function and promote the recovery of prostate.