中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
9期
40-43
,共4页
吴莹%李季倩%孟建%路广林%郝钰
吳瑩%李季倩%孟建%路廣林%郝鈺
오형%리계천%맹건%로엄림%학옥
小檗碱%病毒性肺炎%流感病毒%细胞间黏附分子-1%血管细胞黏附分子-1%炎性细胞%小鼠
小檗堿%病毒性肺炎%流感病毒%細胞間黏附分子-1%血管細胞黏附分子-1%炎性細胞%小鼠
소벽감%병독성폐염%류감병독%세포간점부분자-1%혈관세포점부분자-1%염성세포%소서
Berberine%viral pneumonia%influenza virus%ICAM-1%VCAM-1%inflammatory cell%mice
目的观察小檗碱对流感病毒性肺炎小鼠肺组织中细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)表达及炎性细胞渗出的影响,探讨其抗炎性损伤作用。方法 BALB/c小鼠108只随机分为正常组、模型组、小檗碱组。模型组和小檗碱组予25μL 50LD50病毒液滴鼻建立流感病毒感染的小鼠肺炎模型,感染后1 h,正常组和模型组予以双蒸水灌胃,小檗碱组每日予药物0.005 g/kg腹腔注射,各组均给药2次/d,连续给药5 d。感染后第2、4、6日分别取材,免疫组化法检测肺组织中 ICAM-1、VCAM-1的表达,对肺泡灌洗液(BALF)中白细胞进行分类计数。结果病毒感染后第2、4、6日,模型组肺组织中ICAM-1、VCAM-1的表达明显增强,小檗碱降低了ICAM-1、VCAM-1的表达水平,与模型组比较差异有统计学意义(P<0.01);模型组BALF中白细胞总数、单个核细胞数、嗜酸性粒细胞数、中性粒细胞数均明显升高,小檗碱组第6日BALF中白细胞总数、中性粒细胞数明显降低(P<0.01),第4日时BALF中单个核细胞数降低(P<0.01)。结论小檗碱能够抑制流感病毒性肺炎小鼠肺组织中ICAM-1、VCAM-1表达,减轻了炎性细胞渗出,对小鼠流感病毒性肺炎肺组织炎性损伤具有保护作用。
目的觀察小檗堿對流感病毒性肺炎小鼠肺組織中細胞間黏附分子-1(ICAM-1)、血管細胞黏附分子-1(VCAM-1)錶達及炎性細胞滲齣的影響,探討其抗炎性損傷作用。方法 BALB/c小鼠108隻隨機分為正常組、模型組、小檗堿組。模型組和小檗堿組予25μL 50LD50病毒液滴鼻建立流感病毒感染的小鼠肺炎模型,感染後1 h,正常組和模型組予以雙蒸水灌胃,小檗堿組每日予藥物0.005 g/kg腹腔註射,各組均給藥2次/d,連續給藥5 d。感染後第2、4、6日分彆取材,免疫組化法檢測肺組織中 ICAM-1、VCAM-1的錶達,對肺泡灌洗液(BALF)中白細胞進行分類計數。結果病毒感染後第2、4、6日,模型組肺組織中ICAM-1、VCAM-1的錶達明顯增彊,小檗堿降低瞭ICAM-1、VCAM-1的錶達水平,與模型組比較差異有統計學意義(P<0.01);模型組BALF中白細胞總數、單箇覈細胞數、嗜痠性粒細胞數、中性粒細胞數均明顯升高,小檗堿組第6日BALF中白細胞總數、中性粒細胞數明顯降低(P<0.01),第4日時BALF中單箇覈細胞數降低(P<0.01)。結論小檗堿能夠抑製流感病毒性肺炎小鼠肺組織中ICAM-1、VCAM-1錶達,減輕瞭炎性細胞滲齣,對小鼠流感病毒性肺炎肺組織炎性損傷具有保護作用。
목적관찰소벽감대류감병독성폐염소서폐조직중세포간점부분자-1(ICAM-1)、혈관세포점부분자-1(VCAM-1)표체급염성세포삼출적영향,탐토기항염성손상작용。방법 BALB/c소서108지수궤분위정상조、모형조、소벽감조。모형조화소벽감조여25μL 50LD50병독액적비건립류감병독감염적소서폐염모형,감염후1 h,정상조화모형조여이쌍증수관위,소벽감조매일여약물0.005 g/kg복강주사,각조균급약2차/d,련속급약5 d。감염후제2、4、6일분별취재,면역조화법검측폐조직중 ICAM-1、VCAM-1적표체,대폐포관세액(BALF)중백세포진행분류계수。결과병독감염후제2、4、6일,모형조폐조직중ICAM-1、VCAM-1적표체명현증강,소벽감강저료ICAM-1、VCAM-1적표체수평,여모형조비교차이유통계학의의(P<0.01);모형조BALF중백세포총수、단개핵세포수、기산성립세포수、중성립세포수균명현승고,소벽감조제6일BALF중백세포총수、중성립세포수명현강저(P<0.01),제4일시BALF중단개핵세포수강저(P<0.01)。결론소벽감능구억제류감병독성폐염소서폐조직중ICAM-1、VCAM-1표체,감경료염성세포삼출,대소서류감병독성폐염폐조직염성손상구유보호작용。
Objective To observe the effects of berberine on ICAM-1, VCAM-1 expression and inflammatory cells exudation in mice with viral pneumonia caused by influenza virus, and explore its anti-injury effect. Methods Totally 108 BALB/c mice were randomly divided into control group, model group, and berberine group. 25 μL 50 LD50 influenza virus, mouse lung-adapted strain, was intranasally inoculated to model group and berberine group. 1 h after infection, control and model group were intragastrically given 25 μL distilled water, berberine group was treated by intraperitoneal injection with berberine at a dose of 0.005 g/(kg·d) for 5 days, twice per day. On day 2, 4 and 6 after infection, immunocytochemical method was used to detect ICAM-1 and VCAM-1 expression, and sorting cell count of leukocytes in bronchoalveolar lavage fluid (BALF) were counted. Results The expression of ICAM-1 and VCAM-1 in model group increased obviously on day 2, 4, 6, and which in berberine group decreased compared with model group (P<0.01). WBC, mononuclear cell, eosinophile cell and neutrophil cell number in model group increased significantly. WBC and neutrophil cell number decreased in berberine group on day 6 (P<0.01), and the mononuclear cell number decreased on day 4 (P<0.01). Conclusion Berberine inhibited the expression of ICAM-1 and VCAM-1, and decreased the inflammatory cells exudation in lung of mice with viral pneumonia caused by influenza virus. Berberine has protective effect on inflammatory injury of lung tissue in mice with viral pneumonia caused by influenza virus.