中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
9期
37-39
,共3页
动脉粥样硬化斑块%软脉灵口服液%ApoE基因敲除小鼠%血管新生
動脈粥樣硬化斑塊%軟脈靈口服液%ApoE基因敲除小鼠%血管新生
동맥죽양경화반괴%연맥령구복액%ApoE기인고제소서%혈관신생
atherosclerotic plaque%Ruanmailing Oral Liquid%Apolipoprotein E gene knock-out mice%angiogenesis
目的观察软脉灵口服液对ApoE基因敲除小鼠动脉粥样硬化斑块内血管新生的影响,探讨其稳定斑块的机制。方法30只6～8周龄ApoE基因敲除小鼠,饲以高脂饲料12周后,随机分为模型组、软脉灵组、辛伐他汀组,另以6只正常C57BL/6J小鼠作为对照组。分别给药12周后,小鼠眼眶静脉采血测定胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C),HE染色观察小鼠主动脉病理形态学变化,免疫组化法测定以CD105标记的斑块内微血管密度(MVD)及小鼠主动脉CD105表达。结果与模型组比较,软脉灵组、辛伐他汀组TC、LDL-C、TG 显著降低,而 HDL-C 显著升高(P＜0.01)；且病理损伤程度减轻,MVD 降低,CD105蛋白表达显著降低(P＜0.01)。软脉灵组与辛伐他汀组比较,上述指标差异无统计学意义(P＞0.05)。结论软脉灵口服液可能通过降低斑块内CD105蛋白表达,抑制血管新生,达到稳定动脉粥样硬化斑块目的。
目的觀察軟脈靈口服液對ApoE基因敲除小鼠動脈粥樣硬化斑塊內血管新生的影響,探討其穩定斑塊的機製。方法30隻6～8週齡ApoE基因敲除小鼠,飼以高脂飼料12週後,隨機分為模型組、軟脈靈組、辛伐他汀組,另以6隻正常C57BL/6J小鼠作為對照組。分彆給藥12週後,小鼠眼眶靜脈採血測定膽固醇(TC)、三酰甘油(TG)、低密度脂蛋白膽固醇(LDL-C)、高密度脂蛋白膽固醇(HDL-C),HE染色觀察小鼠主動脈病理形態學變化,免疫組化法測定以CD105標記的斑塊內微血管密度(MVD)及小鼠主動脈CD105錶達。結果與模型組比較,軟脈靈組、辛伐他汀組TC、LDL-C、TG 顯著降低,而 HDL-C 顯著升高(P＜0.01)；且病理損傷程度減輕,MVD 降低,CD105蛋白錶達顯著降低(P＜0.01)。軟脈靈組與辛伐他汀組比較,上述指標差異無統計學意義(P＞0.05)。結論軟脈靈口服液可能通過降低斑塊內CD105蛋白錶達,抑製血管新生,達到穩定動脈粥樣硬化斑塊目的。
목적관찰연맥령구복액대ApoE기인고제소서동맥죽양경화반괴내혈관신생적영향,탐토기은정반괴적궤제。방법30지6～8주령ApoE기인고제소서,사이고지사료12주후,수궤분위모형조、연맥령조、신벌타정조,령이6지정상C57BL/6J소서작위대조조。분별급약12주후,소서안광정맥채혈측정담고순(TC)、삼선감유(TG)、저밀도지단백담고순(LDL-C)、고밀도지단백담고순(HDL-C),HE염색관찰소서주동맥병리형태학변화,면역조화법측정이CD105표기적반괴내미혈관밀도(MVD)급소서주동맥CD105표체。결과여모형조비교,연맥령조、신벌타정조TC、LDL-C、TG 현저강저,이 HDL-C 현저승고(P＜0.01)；차병리손상정도감경,MVD 강저,CD105단백표체현저강저(P＜0.01)。연맥령조여신벌타정조비교,상술지표차이무통계학의의(P＞0.05)。결론연맥령구복액가능통과강저반괴내CD105단백표체,억제혈관신생,체도은정동맥죽양경화반괴목적。
Objective To observe the effect of Ruanmailing Oral Liquid on angiogenesis in atherosclerosis plaque of Apolipoprotein E (ApoE) gene knock-out mice, and explore the mechanisms of plaque stabilizing. Methods Totally 30 mice 6-8 weeks old ApoE knockout mice were fed a high fat diet for 12 weeks until the formation of a mature atherosclerotic plaque. They were randomly divided into three groups-model group, Ruanmailing group, simvastatin group, with another 6 normal C57BL/6J mice as the control group, and were administered for 12 weeks. Blood was extracted from orbital venous to measure the lipid (TC, TG, LDL-C, HDL-C) variation. HE-stain was used to observe aortic pathomorphological changes, meanwhile, immunohistochemical method was adopted to determine the microvessel density of plagues which is marked by CD105, as well as the expression of CD105 in aorta. Results Compared with the model group, TC, LDL-C, TG of Ruanmailing group and simvastatin group decreased significantly, while HDL-C increased significantly (P<0.01), degree of pathological damage was reduced, microvessel density and the expression of CD105 was significantly lower (P<0.01). There was no significant difference between Ruanmailing group and simvastatin group in above-mentioned indicators (P>0.05). Conclusion Ruanmailing Oral Liquid may reduce the expression of CD105 and inhibit the angiogenesis within the plaque, which is the possible mechanism of stabilizing the atherosclerotic plaque.
