CAJ | 학술논문

　　目的观察万寿菊叶黄素抗氧化作用的量效关系,为临床使用剂量提供实验数据。方法小鼠随机分为空白组、模型组及叶黄素1、5、25、125、625 mg/kg组。模型组及各给药组小鼠腹腔注射D-半乳糖120 mg/kg制备氧化损伤模型,空白组注射等体积生理盐水溶液,造模期间各组动物给予相应药物,连续造模7周。测定血清中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性,观察受试物的量效关系。结果叶黄素1、5、25 mg/kg剂量组可显著降低模型小鼠血清MDA水平和升高SOD活性,125、625 mg/kg剂量组则未显示出显著的抗氧化活性。结论叶黄素对小鼠D-半乳糖氧化损伤模型抗氧化活性在1～25 mg/kg范围内较为显著,提示临床用量应在此范围内进行选择,不宜超大剂量服用。
　　목적관찰만수국협황소항양화작용적량효관계,위림상사용제량제공실험수거。방법소서수궤분위공백조、모형조급협황소1、5、25、125、625 mg/kg조。모형조급각급약조소서복강주사D-반유당120 mg/kg제비양화손상모형,공백조주사등체적생리염수용액,조모기간각조동물급여상응약물,련속조모7주。측정혈청중병이철(MDA)수평화초양화물기화매(SOD)활성,관찰수시물적량효관계。결과협황소1、5、25 mg/kg제량조가현저강저모형소서혈청MDA수평화승고SOD활성,125、625 mg/kg제량조칙미현시출현저적항양화활성。결론협황소대소서D-반유당양화손상모형항양화활성재1～25 mg/kg범위내교위현저,제시림상용량응재차범위내진행선택,불의초대제량복용。
Objective To observe the antioxidative dosage-effect relationship of Marigold lutein, and provide experimental data for clinical use. Methods The mice were randomly divided into seven groups:blank control group, model control group, 1 mg/kg lutein group, 5 mg/kg lutein group, 25 mg/kg lutein group, 125 mg/kg lutein group and 625 mg/kg lutein group. The mice in blank control group were dealt with saline solution by intraperitoneal injection, the others were dealt with D-galactose (120 mg/kg) by intraperitoneal injection for seven weeks to make oxidative damage model, meanwhile the mice were given corresponding dose of the drug. Subsequently, the level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in the serum were measured, and the antioxidative dosage-effect relationship was observed. Results The 1, 5, 25 mg/kg lutein reduced the MDA level and increased SOD activity, and the 125, 625 mg/kg dose of lutein did not show significant antioxidant activity. Conclusion Lutein has significant antioxidant activity in mouse dealt with D-galactose within the dose range of 1-25 mg/kg. The results suggest that the clinical dosage range of lutein should be kept within reasonable limits.