南昌大学学报（医学版）
南昌大學學報（醫學版）
남창대학학보（의학판）
ACTA ACADEMIAE MEDICINAE JIANGXI
2014年
2期
17-19,107
,共4页
程书榜%李林林%朱量%秦颖
程書榜%李林林%硃量%秦穎
정서방%리림림%주량%진영
NF-κB%热休克蛋白 70%急性坏死性胰腺炎%机制%实验,动物%大鼠
NF-κB%熱休剋蛋白 70%急性壞死性胰腺炎%機製%實驗,動物%大鼠
NF-κB%열휴극단백 70%급성배사성이선염%궤제%실험,동물%대서
nuclear factor-κB%heat shock protein 70%acute necrotizing pancreatitis%mechanism%animals,laboratory%rats
目的：探讨热休克蛋白70（heat shock protein 70，Hsp70）减轻大鼠急性坏死性胰腺炎的机制。方法将60只 SD 大鼠按随机数字表法分为3组，每组20只。A 组急性坏死性胰腺炎（aute nicrosis apoptonisis，ANP）模型由5％牛黄胆酸钠大鼠胰胆管逆行注射诱发而成，B 组手术进腹后翻动十二指肠，C 组使用温生理盐水持续腹腔灌洗30 min，10 h 后同 A 组造 ANP 模型。于最后1次手术后6 h 处死大鼠，检查各组大鼠血清细胞因子 IL-6、TNF-α和 IL-8及血清淀粉酶；取胰腺组织行病理检查，并分别使用 PCR 和免疫组织化学方法检测胰腺中 Hsp70和 NF-κB 的表达。结果C 组大鼠血清淀粉酶为（2845．70±204．78）U·L-1，明显低于 A 组的（4064．93±188．97）U· L-1，高于 B 组的（1221．47±54．58）U·L-1（均 P ＜0．05）；C 组大鼠血清 TNF-α、IL-6、IL-8明显低于 A 组，高于B 组（均 P ＜0．05）；C 组大鼠胰腺组织 Hsp70 mRNA RQ 值明显低于 A 组，但高于 B 组（P ＜0．05）；NF-κB 在胰腺中的表达：A 组部分细胞核强阳性染色，胞浆中弱阳性染色，B、C 组细胞核染色明显减弱，胞浆则未见染色。结论HSP70可以通过调控 NF-κB 从而下调细胞因子的表达，进而改善大鼠 ANP 病理生理。
目的：探討熱休剋蛋白70（heat shock protein 70，Hsp70）減輕大鼠急性壞死性胰腺炎的機製。方法將60隻 SD 大鼠按隨機數字錶法分為3組，每組20隻。A 組急性壞死性胰腺炎（aute nicrosis apoptonisis，ANP）模型由5％牛黃膽痠鈉大鼠胰膽管逆行註射誘髮而成，B 組手術進腹後翻動十二指腸，C 組使用溫生理鹽水持續腹腔灌洗30 min，10 h 後同 A 組造 ANP 模型。于最後1次手術後6 h 處死大鼠，檢查各組大鼠血清細胞因子 IL-6、TNF-α和 IL-8及血清澱粉酶；取胰腺組織行病理檢查，併分彆使用 PCR 和免疫組織化學方法檢測胰腺中 Hsp70和 NF-κB 的錶達。結果C 組大鼠血清澱粉酶為（2845．70±204．78）U·L-1，明顯低于 A 組的（4064．93±188．97）U· L-1，高于 B 組的（1221．47±54．58）U·L-1（均 P ＜0．05）；C 組大鼠血清 TNF-α、IL-6、IL-8明顯低于 A 組，高于B 組（均 P ＜0．05）；C 組大鼠胰腺組織 Hsp70 mRNA RQ 值明顯低于 A 組，但高于 B 組（P ＜0．05）；NF-κB 在胰腺中的錶達：A 組部分細胞覈彊暘性染色，胞漿中弱暘性染色，B、C 組細胞覈染色明顯減弱，胞漿則未見染色。結論HSP70可以通過調控 NF-κB 從而下調細胞因子的錶達，進而改善大鼠 ANP 病理生理。
목적：탐토열휴극단백70（heat shock protein 70，Hsp70）감경대서급성배사성이선염적궤제。방법장60지 SD 대서안수궤수자표법분위3조，매조20지。A 조급성배사성이선염（aute nicrosis apoptonisis，ANP）모형유5％우황담산납대서이담관역행주사유발이성，B 조수술진복후번동십이지장，C 조사용온생리염수지속복강관세30 min，10 h 후동 A 조조 ANP 모형。우최후1차수술후6 h 처사대서，검사각조대서혈청세포인자 IL-6、TNF-α화 IL-8급혈청정분매；취이선조직행병리검사，병분별사용 PCR 화면역조직화학방법검측이선중 Hsp70화 NF-κB 적표체。결과C 조대서혈청정분매위（2845．70±204．78）U·L-1，명현저우 A 조적（4064．93±188．97）U· L-1，고우 B 조적（1221．47±54．58）U·L-1（균 P ＜0．05）；C 조대서혈청 TNF-α、IL-6、IL-8명현저우 A 조，고우B 조（균 P ＜0．05）；C 조대서이선조직 Hsp70 mRNA RQ 치명현저우 A 조，단고우 B 조（P ＜0．05）；NF-κB 재이선중적표체：A 조부분세포핵강양성염색，포장중약양성염색，B、C 조세포핵염색명현감약，포장칙미견염색。결론HSP70가이통과조공 NF-κB 종이하조세포인자적표체，진이개선대서 ANP 병리생리。
Objective To explore the mechanism of action of heat shock protein 70(Hsp70)in relieving acute necrotizing pancreatitis(ANP).Methods Sixty SD rats were randomly divided in-to three groups,with 20 rats in each group.In group A,ANP was induced by retrograde injection of 5% sodium taurocholate into pancreatic duct.In group B,the duodenum was flipped after ab-dominal surgery for the sham operation.In group C,ANP was induced 10 hours after continuous peritoneal lavage with warm saline for 30 minutes.The rats were sacrificed 6 hours after the last operation.Serum levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8)and amylase were measured by ELISA.In addition,pancreas tissues were obtained to examine the pathological changes.HSP70 expression was examined by PCR and nuclear factor-κB(NF-κB) expression by immunohistochemistry.Results The levels of serum amylase in group C[(2 845.70± 204.78)U·L-1 )]were lower than those in group A[(4 064.93±188.97)U·L-1 ],but higher than those in group B[(1 221.47±54.58 )U·L-1 ](all P <0.05);The levels of serum TNF-α, IL-6 and IL-8 in group C were lower than those in group A,but higher than those in group B(all P <0.05);The RQ of Hsp70 mRNA in group C was lower than that in group A,but higher than that in group B(P < 0.05);Immunohistochemistry showed strong positive nuclear staining and weak positive cytoplasmic staining for NF-κB in group A,and weak positive nuclear staining and negative cytoplasmic staining for NF-κB in group B and C.Conclusion HSP70 can decrease cyto-kine expression and reduce pathophysiological damage through down-regulating the expression of NF-κB in ANP rats.
