CAJ | 학술논문

目的建立一种优化的体外成人成肌细胞分离与原代培养方法.方法首先用两步消化法对取自成人骨骼肌组织进行消化获取相对较纯的成肌细胞,通过差速贴壁法进行进一步的纯化,并对获取的成肌细胞进行细胞形态学观察、免疫组织化学、透射电镜鉴定及生长曲线的绘制与分析.结果该方法可获取高纯度的成肌细胞,且在体外可快速稳定增殖.结论该方法所获取的成肌细胞可为Duchenne肌营养不良的治疗、血友病、血栓闭塞性脉管炎(伯格氏病)、心肌梗死和慢性心衰的基因治疗的研究提供一种充足、可靠的实验靶细胞.
목적 건립일충우화적체외성인성기세포분리여원대배양방법.방법 수선용량보소화법대취자성인골격기조직진행소화획취상대교순적성기세포,통과차속첩벽법진행진일보적순화,병대획취적성기세포진행세포형태학관찰、면역조직화학、투사전경감정급생장곡선적회제여분석.결과 해방법가획취고순도적성기세포,차재체외가쾌속은정증식.결론 해방법소획취적성기세포가위Duchenne기영양불량적치료、혈우병、혈전폐새성맥관염(백격씨병)、심기경사화만성심쇠적기인치료적연구제공일충충족、가고적실험파세포.
Objective To establish an optimal method for in vitro culture and amplification of myoblasts from human skeletal muscle. Methods The myoblasts were isolated from human skeletal muscle with two steps combined dissociation, then the harvested myoblasts were purified by the way of different attachment. The cultured cells were identified by morphology, immunohistochemistry and transmission e-lectron microscopy. Growth curves were plotted and analyzed. Results A lot of highly pure myoblasts could be obtained by this procedure and cultured in vitro steadily and rapidly. Conclusion It shows promise for the treatment of DMD, hemophilia, Buergers disease, myocar-dial infarction and chronic heart failure.