CAJ | 학술논문

目的：：制备辣椒碱脂质体及通过体外透皮试验研究其可行性。方法：采用薄膜-超声法制备辣椒碱脂质体,以包封率为指标,在单因素试验基础上采用正交试验法,筛选最优处方。采用改良Franz扩散池进行透皮试验,比较辣椒碱混悬液、辣椒碱脂质体和辣椒碱软膏经大鼠离体皮肤的累积透过量。结果：辣椒碱脂质体的最佳处方：药脂比1∶5,100 mg吐温-80,50 mg维生素E,10 ml二氯甲烷,10 ml外水相PBS及pH 6.5,超声8 min。透皮试验12 h累积透过率：辣椒碱脂质体>辣椒碱-PBS混悬液>辣椒碱软膏；辣椒碱脂质体12 h累积透过量最多,且呈现明显的缓释效果。结论：所制备的辣椒碱脂质体包封率高,并具有良好透皮作用和缓释作用,该工艺简便易行。
목적：：제비랄초감지질체급통과체외투피시험연구기가행성。방법：채용박막-초성법제비랄초감지질체,이포봉솔위지표,재단인소시험기출상채용정교시험법,사선최우처방。채용개량Franz확산지진행투피시험,비교랄초감혼현액、랄초감지질체화랄초감연고경대서리체피부적루적투과량。결과：랄초감지질체적최가처방：약지비1∶5,100 mg토온-80,50 mg유생소E,10 ml이록갑완,10 ml외수상PBS급pH 6.5,초성8 min。투피시험12 h루적투과솔：랄초감지질체>랄초감-PBS혼현액>랄초감연고；랄초감지질체12 h루적투과량최다,차정현명현적완석효과。결론：소제비적랄초감지질체포봉솔고,병구유량호투피작용화완석작용,해공예간편역행。
Objective:To prepare capsaicin liposomes and study the feasibility by in vitro percutaneous penetration test. Meth-ods:Capsaicin liposomes were prepared by a film-ultrasonic method. The best formula was screened by orthogonal test based on single factor studies with the entrapment efficiency as the index. The improved Franz diffusion cells were used to study the transdermal pene-tration of capsaicin suspensions, capsaicin liposomes and capsaicin ointments, and the cumulative penetration amount through the isola-ted rat skin was compared. Results:The optimal formula of capsaicin liposomes were as follows:the ratio of capsaicin to lipids was 1∶5;the amount of Tween-80 was 100 mg;the amount of vitamin E was 50 mg;10 ml dichloromethane was used as the solvent. The op-timal pH value of the external phase was 6. 5 with the volume of 10 ml. The ultrasonic time was 8 min. The order of 12-hour cumulative penetration amount was capsaicin liposomes>capsaicin-PBS suspensions>capsaicin ointments. Capsaicin liposomes had the highest 12-hour cumulative penetration amount and showed obvious sustained-release property. Conclusion:Capsaicin liposomes have high en-trapment efficiency, good percutaneous penetration and sustained-release property, and the preparation technology is simple.