中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2014年
10期
50-54,58
,共6页
程世杰%刘长海%陈穗%刘占
程世傑%劉長海%陳穗%劉佔
정세걸%류장해%진수%류점
米曲霉%紫外诱变%蛋白酶%广东地区
米麯黴%紫外誘變%蛋白酶%廣東地區
미곡매%자외유변%단백매%엄동지구
Aspergillus oryzae%UV mutation%protease%Guangdong area
以广东地区高盐稀态酱油成曲中优良的米曲霉菌株为出发菌,经紫外诱变选育得到了一株种曲单孢子,中性蛋白酶活力比出发菌株提高了108%,平均种曲酶活力为9426.43 U/g 干基,孢子发芽率达91.58%,成曲中性蛋白酶活力为209 U/g 干基,碱性蛋白酶活力为159 U/g 干基,酸性蛋白酶活力为101 U/g干基,纤维素酶活力为5.3 U/g干基,黄曲霉毒素B1检出量符合国家标准规定,拥有良好遗传稳定性的米曲霉突变菌株。
以廣東地區高鹽稀態醬油成麯中優良的米麯黴菌株為齣髮菌,經紫外誘變選育得到瞭一株種麯單孢子,中性蛋白酶活力比齣髮菌株提高瞭108%,平均種麯酶活力為9426.43 U/g 榦基,孢子髮芽率達91.58%,成麯中性蛋白酶活力為209 U/g 榦基,堿性蛋白酶活力為159 U/g 榦基,痠性蛋白酶活力為101 U/g榦基,纖維素酶活力為5.3 U/g榦基,黃麯黴毒素B1檢齣量符閤國傢標準規定,擁有良好遺傳穩定性的米麯黴突變菌株。
이엄동지구고염희태장유성곡중우량적미곡매균주위출발균,경자외유변선육득도료일주충곡단포자,중성단백매활력비출발균주제고료108%,평균충곡매활력위9426.43 U/g 간기,포자발아솔체91.58%,성곡중성단백매활력위209 U/g 간기,감성단백매활력위159 U/g 간기,산성단백매활력위101 U/g간기,섬유소매활력위5.3 U/g간기,황곡매독소B1검출량부합국가표준규정,옹유량호유전은정성적미곡매돌변균주。
Take an excellent Aspergillus oryzae selected from the koji of Guangdong soy sauce with process of high-salt-diluted-state fermentation as original strain,which is induced mutation by ultraviolet.The result shows that the neutral protease activity of the mutant is 108% higher than that of the original strain;the average enzyme activity of koji reaches 9426.43 U/g (dm),the spore germination rate reaches 91.58%;the soybean koji neutral protease activity reaches 209 U/g (dm),the alkaline protease activity reaches 159 U/g (dm),the acid protease activity reaches 101 U/g (dm),the cellulase activity reaches 5 .3 U/g (dm).The detectable amount of Aflatoxin B1 complies with the requirements of the national standard,and it has good genetic stability.