国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
11期
1003-1006,1011
,共5页
逯素芬%于爱兰%刘献文%张宗旺
逯素芬%于愛蘭%劉獻文%張宗旺
록소분%우애란%류헌문%장종왕
尼古丁%依赖%戒断%痛觉过敏%白细胞介素-1β
尼古丁%依賴%戒斷%痛覺過敏%白細胞介素-1β
니고정%의뢰%계단%통각과민%백세포개소-1β
Nicotine%Dependence%Withdrawal%Hyperalgesia%Interleukin-1β
目的 探讨切口周围组织中白细胞介素(interleukin,IL)-1β在尼古丁戒断大鼠术后切口痛痛觉过敏中的作用.方法 SD大鼠96只,按随机数字表法分为4组:正常对照组(Naive组,6只)、尼古丁戒断组(NT组,30只)、切口痛组(IP组,30只)和尼古丁戒断切口痛组(NT+IP组,30只).NT组、IP组和NT+IP组大鼠按术前2h和术后2、24、48、72 h分为5个亚组,每个亚组6只.观察各组大鼠上述时间点机械刺激缩足阈值(paw mechanical withdrawal threshold,MWT)、热刺激缩足潜伏期(paw thermal withdrawal latency,TWL)和后爪水肿的变化;取大鼠术侧后爪切口周围皮肤组织,应用酶联免疫分析法检测IL-1β含量.结果 与IP组比较,NT+IP组大鼠术后2、24、48、72 h MwT和TWL均降低(P＜0.05),且术侧后爪水肿明显,其中以术后24 h水肿最为显著(P＜0.01).与IP组各时间点比较,NT+IP组大鼠相应时间点切口周围组织中IL-1β水平明显增高(P＜0.01).与术前2 h(19.7±1.9) ng/L比较,NT+IP组大鼠切口周围组织中IL-1β水平术后各时间点均明显升高(P＜0.01);术后2h(62.2±3.7) ng/L开始升高,至术后48 h(129.5±6.3) ng/L达高峰,术后72 h开始回降.结论 尼古丁戒断引起大鼠术后痛觉敏感性增高,局部水肿明显;IL-1β参与了尼古丁戒断大鼠术后切口痛痛觉过敏.
目的 探討切口週圍組織中白細胞介素(interleukin,IL)-1β在尼古丁戒斷大鼠術後切口痛痛覺過敏中的作用.方法 SD大鼠96隻,按隨機數字錶法分為4組:正常對照組(Naive組,6隻)、尼古丁戒斷組(NT組,30隻)、切口痛組(IP組,30隻)和尼古丁戒斷切口痛組(NT+IP組,30隻).NT組、IP組和NT+IP組大鼠按術前2h和術後2、24、48、72 h分為5箇亞組,每箇亞組6隻.觀察各組大鼠上述時間點機械刺激縮足閾值(paw mechanical withdrawal threshold,MWT)、熱刺激縮足潛伏期(paw thermal withdrawal latency,TWL)和後爪水腫的變化;取大鼠術側後爪切口週圍皮膚組織,應用酶聯免疫分析法檢測IL-1β含量.結果 與IP組比較,NT+IP組大鼠術後2、24、48、72 h MwT和TWL均降低(P＜0.05),且術側後爪水腫明顯,其中以術後24 h水腫最為顯著(P＜0.01).與IP組各時間點比較,NT+IP組大鼠相應時間點切口週圍組織中IL-1β水平明顯增高(P＜0.01).與術前2 h(19.7±1.9) ng/L比較,NT+IP組大鼠切口週圍組織中IL-1β水平術後各時間點均明顯升高(P＜0.01);術後2h(62.2±3.7) ng/L開始升高,至術後48 h(129.5±6.3) ng/L達高峰,術後72 h開始迴降.結論 尼古丁戒斷引起大鼠術後痛覺敏感性增高,跼部水腫明顯;IL-1β參與瞭尼古丁戒斷大鼠術後切口痛痛覺過敏.
목적 탐토절구주위조직중백세포개소(interleukin,IL)-1β재니고정계단대서술후절구통통각과민중적작용.방법 SD대서96지,안수궤수자표법분위4조:정상대조조(Naive조,6지)、니고정계단조(NT조,30지)、절구통조(IP조,30지)화니고정계단절구통조(NT+IP조,30지).NT조、IP조화NT+IP조대서안술전2h화술후2、24、48、72 h분위5개아조,매개아조6지.관찰각조대서상술시간점궤계자격축족역치(paw mechanical withdrawal threshold,MWT)、열자격축족잠복기(paw thermal withdrawal latency,TWL)화후조수종적변화;취대서술측후조절구주위피부조직,응용매련면역분석법검측IL-1β함량.결과 여IP조비교,NT+IP조대서술후2、24、48、72 h MwT화TWL균강저(P＜0.05),차술측후조수종명현,기중이술후24 h수종최위현저(P＜0.01).여IP조각시간점비교,NT+IP조대서상응시간점절구주위조직중IL-1β수평명현증고(P＜0.01).여술전2 h(19.7±1.9) ng/L비교,NT+IP조대서절구주위조직중IL-1β수평술후각시간점균명현승고(P＜0.01);술후2h(62.2±3.7) ng/L개시승고,지술후48 h(129.5±6.3) ng/L체고봉,술후72 h개시회강.결론 니고정계단인기대서술후통각민감성증고,국부수종명현;IL-1β삼여료니고정계단대서술후절구통통각과민.
Objective To explore the contribution of interleukin (IL)-1β in surrounding incision tissues to postoperative hyperalgesia in rats with nicotine withdrawal.Methods Ninety-six SD rats were randomly divided into Naive group (n=6),NT group (n=30),IP group (n=30) and NT+IP group (n=30).The rats in NT group,IP group and NT+IP group were divided into 5 subgroups by preoperative 2 h,postoperative 2,24,48 h and 72 h,six in each subgroup.Both paw mechanical withdrawal threshold (MWT) and paw thermal withdrawal latency (TWL) were determined at above time points for every rats.The hind paw edema was also observed.The level of IL-1β in the surrounding incisional tissues was measured by Elisa method.Results Compared with IP group,foot plantar MWT and TWL in NT+IP group were significantly reduced at postoperative 2,24,48 h and 72 h (P＜0.05),with most significant decrease at postoperative 48 h (P＜0.01).Compared with IP group for each time point,IL-1β level in NT+IP group was significantly increased (P＜0.01).Compared with preoperative 2 h (19.7± 1.9) ng/L,IL-1β level at all the time points in NT+IP group were significantly increased (P＜0.01).The IL-1β level began to rise at postoperative 2 h (62.2±3.7) ng/L,peaked at postoperative 48 h (129.5±6.3) ng/L,and then decreased at postoperative 72 h.Conclusions Nicotine withdrawal can cause postoperative incisional hyperalgesia and hind paw edema in rats.IL-1β is involved in postoperative incisional hyperalgesia in rats with nicotine abstinence.
