CAJ | 학술논문

本研究通过自制牛源荚膜血清A型多杀性巴氏杆菌灭活菌苗免疫产蛋鸡，采用卡拉胶结合硫酸铵沉淀法提取卵黄抗体IgY，并采用间接血凝方法检测抗体效价。结果表明，抗原致敏红细胞的最佳浓度是800μg/mL，免疫后第7周抗体效价达到高峰，效价为1：1024，高效价持续5周开始下降，测定提取的IgY浓度为8.258mg/mL，无菌检测及动物安全性实验表明制备的卵黄抗体安全可靠。本研究制备了抗牛多杀性巴氏杆菌卵黄抗体，为防治由荚膜血清A型多杀性巴氏杆菌所致的犊牛肺炎提供了新的手段。
본연구통과자제우원협막혈청A형다살성파씨간균멸활균묘면역산단계，채용잡랍효결합류산안침정법제취란황항체IgY，병채용간접혈응방법검측항체효개。결과표명，항원치민홍세포적최가농도시800μg/mL，면역후제7주항체효개체도고봉，효개위1：1024，고효개지속5주개시하강，측정제취적IgY농도위8.258mg/mL，무균검측급동물안전성실험표명제비적란황항체안전가고。본연구제비료항우다살성파씨간균란황항체，위방치유협막혈청A형다살성파씨간균소치적독우폐염제공료신적수단。
By the preparation and establishment of the indirect hemagglutination of highly yolk antibody against bovine capsular serotype A pasteurella multocida(Pm), the hens were immunized with the inactivated vaccine of bovine Pm. The antibody titer was tested by indirect hemagglutination, IgY were extracted by carrageenan and salt precipitation with ammonium sulfate. The result showed that the best antigen concentration was 800μg/mL, IgY antibody titer reached the peak in the 7th week after the first immunized and titer of 1:1024 and last for 5 weeks. The IgY concentration obtained from egg yolk was 8.258mg/mL. Sterility detection and animals safe experiment results showed that the egg yolk antibodies were safe. Against bovine pasteurella multocida IgY was prepared, it also provided a new immune preparations for the prevention and control of the capsular serotype A pasteurella multocida pneumonia in calves.